A kind of Bacillus subtilis and its application in the preparation of antibacterial peptides and feed

A technology of Bacillus subtilis and iturin, applied in the field of microorganisms, can solve the problems of inability to prevent and treat necrotic enteritis, no inhibitory effect of Clostridium perfringens, single antibacterial peptide, etc., and achieve improvement of poultry intestinal tract Road health, treatment and prevention of necrotic enteritis, effects of fewer procedures

Active Publication Date: 2022-07-26
安杰利(重庆)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the antibacterial peptides produced by Bacillus subtilis are single, and most of them have no inhibitory effect or weak inhibitory effect on Clostridium perfringens, and cannot prevent and treat necrotic enteritis

Method used

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  • A kind of Bacillus subtilis and its application in the preparation of antibacterial peptides and feed
  • A kind of Bacillus subtilis and its application in the preparation of antibacterial peptides and feed
  • A kind of Bacillus subtilis and its application in the preparation of antibacterial peptides and feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] 1. Screening of Bacillus subtilis against Clostridium:

[0040] (1) Collect samples of fermented food, micro-ecological preparations, etc., and place them in nutrient broth medium at 1:100 and shake them on a shaker for 12 hours.

[0041](2) Dilute and spread the cultured nutrient broth in a gradient manner, and coat the nutrient agar plate to grow between 30-300 single colonies. A single colony was cultured in a 96-well plate (100 μL of nutrient broth medium was placed in each well of the plate), and the culture was continued for 12 hours.

[0042] (3) Centrifuge the cultured 96-well plate, take the supernatant, and set aside

[0043] (4) Culture Clostridium perfringens CVCC2027 anaerobically in BHI medium for 12 hours

[0044] (5) After sterilizing the TSC medium, incubate at 55°C, then add the cultured Clostridium perfringens to the TSC medium at 1:100, shake well, and pour the medium into a petri dish

[0045] (6) After the petri dish is solidified, punch holes w...

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Abstract

The present invention provides a Bacillus subtilis, which is characterized in that: the bacteria is preserved in the General Microorganism Center of the China Microorganism Culture Collection Management Committee, the preservation address is Beijing, China, the preservation date is November 03, 2020, and the preservation number is CGMCC No. .20997. The Bacillus subtilis can secrete three specific antimicrobial peptides, and the Bacillus subtilis and its metabolites have dual effects of antibacterial and anti-inflammatory. The present invention also provides various uses of the Bacillus subtilis.

Description

technical field [0001] The invention relates to a strain of Bacillus subtilis and its metabolites, belonging to the field of microorganisms. [0002] technical background [0003] Clostridium perfringens, also known as Clostridium perfringens, is the most common Clostridium in clinical gas gangrene pathogens. It is widely distributed in nature and exists in the intestines of many healthy animals. When feeding and environmental conditions change, it can cause Clostridium perfringens disease in livestock and poultry. In the field of poultry and sow farming, C. perfringens is an important pathogenic bacteria that endangers animal health, and it is divided into three grades: when C. perfringens in the gut <10 4 CFU / g, does not affect the production performance and health level of animals, when it is at 10 4 -10 6 CFU / g, the animal is in a subclinical state, without obvious pathological symptoms, but its production performance is reduced, manifested as a reduction in feed co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/02C12P21/04A23K10/18A23K20/147A23K10/12A23K10/16A23K50/75C12R1/125
CPCC12N1/20C12P21/02C07K7/06C07K7/64C07K7/08A23K10/18A23K20/147A23K10/12A23K10/16A23K50/75
Inventor 张江丁修良
Owner 安杰利(重庆)生物科技有限公司
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