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Vaccine for preventing or treating congenital infection with cytomegalovirus

A human cytomegalovirus, congenital technology, applied in the field of vaccines for the prevention or treatment of congenital infection of cytomegalovirus, capable of addressing the effects of transplacental infection No hints were given, and the combination use could not be accurately shown effect, the effect of the infection defense capability has not yet been confirmed, etc.

Active Publication Date: 2021-07-23
KM生物医薬股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] Regarding the effect of the combined use of pentamer and gB, Patent Document 1 reports that the combined use of pentamer and gB is effective in an infection defense test in monkeys, but the effect on transplacental infection is not given. any revelation
In addition, although the combined use group of pentamer + gB was shown to be superior to the pentamer alone group and the non-immune group, the effect of the combination cannot be accurately shown because the gB alone group was not set
[0019] In addition, in Non-Patent Document 12, the effect of combined use of anti-gB monoclonal antibody and anti-pentamer monoclonal antibody was verified in vitro. Although it is believed that the combined use has advantages in terms of neutralization ability and suppression of drug-resistant strains, it has not yet been established. Demonstrating the effect of combination use on infection defense capabilities in organisms
[0020] Furthermore, Patent Document 2 gives the data that immunization with a gB+pentamer bivalent vaccine can increase the production of certain cytokines higher than that of the individual group, but the combined use group has no advantage in terms of neutralization ability, and no infection experiments

Method used

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  • Vaccine for preventing or treating congenital infection with cytomegalovirus
  • Vaccine for preventing or treating congenital infection with cytomegalovirus
  • Vaccine for preventing or treating congenital infection with cytomegalovirus

Examples

Experimental program
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Effect test

Embodiment

[0101] [Materials / Methods]

[0102]

[0103] For evaluation using a guinea pig transplacental infection model system, guinea pig cytomegalovirus (GPCMV) showing infectivity to guinea pigs was used. Since the recombinant GPCMV gB protein sometimes contains aggregates, a modified GPCMV gB protein that does not contain aggregates and has improved properties was produced.

[0104] Artificially synthesized the gene encoding gB (SEQ ID NO. 15) with a leader sequence added to the ectodomain (1-656aa) of gB derived from GPCMV 22122 strain, and amino acid mutations were introduced to improve the traits, and cloned into pCAGGS1-dhfr-neo (Patent Document 3). It is designed to have a His-tag added to the C-terminus of gB. For expression, the Expi293 expression system (Life Technologies Ltd.) was used. The expression plasmid was transfected into the cells, and the cultured supernatant for 4 to 6 days was recovered. From the culture supernatant containing GPCMVgB, it was purified usin...

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PUM

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Abstract

The problem addressed by the present invention is to provide an effective vaccine for preventing or treating congenital infection with CMV. This vaccine for preventing or treating congenital infection with cytomegalovirus (CMV) contains a CMV envelope glycoprotein B (gB protein) antibody and a pentamer antibody.

Description

technical field [0001] The present invention relates to a vaccine for preventing or treating congenital infection with cytomegalovirus. Background technique [0002] There are two main types of cytomegalovirus (CMV) infection. The first type is congenital CMV infection that occurs in the fetus when a pregnant woman is first infected, and the second type is CMV pneumonia, enteritis, retinitis, etc., that occurs in immunosuppressed patients such as transplantation, AIDS, and congenital immunodeficiency disease organ damage. Among them, congenital CMV infection is one of TORCH syndromes, and is an important congenital infection that causes fetal malformation or severe clinical symptoms. When pregnant women are infected with CMV for the first time, about 40% of them pass the placenta and congenital infection occurs to the fetus (in this specification, the term "congenital infection" and the term "transplacental infection" are used in the same meaning). In addition, it has als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61P31/22A61P37/04C07K14/045
CPCA61P31/22A61K39/12C12N2710/16134A61K2039/575A61K2039/545A61K2039/55561C07K14/005C12N2710/16122A61K2039/70A61K2039/55A61K2039/55505Y02A50/30A61K39/245A61P37/04A61P31/20A61K2039/552C12N7/00
Inventor 鸟饲正治森泰亮西村知裕片山贵裕枦山纮辅松本美幸清水裕之井上直树
Owner KM生物医薬股份公司