Continuous reaction device based on nucleic acid extraction, purification and amplification

Abstract

The invention relates to a continuous reaction device based on nucleic acid extraction, purification and amplification. The device comprises a nucleic acid extraction device, a nucleic acid purification device and a nucleic acid amplification reaction device, the nucleic acid extraction device comprises a sample adding layer and a pipeline layer, the nucleic acid purification device comprises an ultrasonic unit and a magnetic suction unit, and the nucleic acid amplification reaction device comprises a temperature control unit and an optical detection unit; the nucleic acid extraction device is used for cracking the sample to obtain a mixture of nucleic acid and protein; the nucleic acid purification device is used for independently separating nucleic acid from the mixture; the nucleic acid amplification reaction device is used for amplifying nucleic acid at a specified temperature by using the temperature control unit; the sample adding layer is arranged above the pipeline layer, the ultrasonic unit and the magnetic attraction unit are arranged below the purification bin in the pipeline layer, the temperature control unit is arranged below the amplification bin in the pipeline layer, and the optical detection unit is arranged on the side face and above the pipeline layer. The device realizes the continuity of the experiment, does not need manual intervention in the process, and is convenient and efficient.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a continuous reaction device based on nucleic acid extraction, purification and amplification. Background technique [0002] The extraction, purification, and amplification of nucleic acid are routine operations in nucleic acid detection tests, and are also critical steps in the process of gene analysis. In the process of gene analysis, it is often necessary to separate specific nucleic acid fragments from mixed samples for subsequent PCR amplification. Therefore, the purification and recovery of nucleic acid directly affects the progress and results of the entire gene analysis process. [0003] The Chinese patent discloses a nucleic acid automatic detection device, which includes a fixed base and a main support plate vertically fixed on the fixed base, an automatic sample reaction mechanism and its fixed frame, a nucleic acid detection optical path structure and its supportin...

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Problems solved by technology

[0004] In the above technical solutions, on the one hand, the detection device has high environmental requirements and low test accuracy. At the same time, the internal pipeline of the chip is not integrated, and it is difficult to mix and test the reaction solution as expected, and the extraction, purification and amplification of nucleic acid usually require Use different instruments to complete, and the operation is cumbersome

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