Automatic bacteriophage panning platform based on digital microfluidic technology

Abstract

The invention discloses an automatic bacteriophage panning platform based on a digital micro-fluidic technology, which comprises a digital micro-fluidic platform, the digital micro-fluidic platform is composed of a micro-fluidic chip, an integrated circuit, a temperature control module and a magnetic separation module, the micro-fluidic chip and the integrated circuit thereof are used for fluid manipulation, the temperature control module is used for providing proper temperature for amplification of bacteria and bacteriophages, and the magnetic separation module is used for realizing integrated magnetic bead washing. Three rounds of biological elutriation can be automatically completed within 16 hours through the automatic elutriation scheme, and compared with complex manual operation of nearly one week in a traditional method, labor force is greatly liberated. In addition, the reagent dosage of the scheme is only 3% of that of a conventional method, so that the reagent cost is greatly reduced. In short, the invention further promotes screening of the affinity polypeptide and application of the affinity polypeptide in the fields of diagnosis, imaging, delivery, treatment and the like.

Description

technical field [0001] The invention relates to an automatic panning platform and panning implementation scheme based on phage display, and belongs to the technical field of digital microfluidics. Background technique [0002] Molecular recognition is the molecular basis of physiological and pathological activities, and most complex physiological and pathological activities depend on molecular recognition. Phage display technology is one of the effective methods to study molecular recognition. Phage display technology inserts foreign genes into the phage genome through genetic engineering, so that foreign peptides or antibodies can be fused and expressed on the surface of the phage (Angew. Chem. Int. Ed. 2019, 58, 14428-14437). The displayed peptides or antibodies on the surface of the phage can be used for affinity screening directly against different targets, and the sequences of the displayed peptides or antibodies can be easily obtained by phage gene sequencing. So far...

AI Technical Summary

Problems solved by technology

[0005] Relying on digital microfluidic technology, the present invention proposes an automated phage panning platform for the problems of high repeatability, time-consuming, labor-intensive operation, and high reagent consumption in traditional phage display technology affinity screening, which is automated and integrated , free labor, less reagent consumption and other advantages

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