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Water chestnut stem tip tissue culture method

A technology of tissue culture and water chestnut stems, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of pest damage, increased control costs, and decreased unit benefits

Pending Publication Date: 2021-08-06
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

In the past ten years, my country's water chestnut industry has developed rapidly. However, for a long time, farmers kept and propagated the bulbs, resulting in serious species degradation, serious damage from diseases and insect pests, a substantial increase in control costs, a significant decrease in yield, and a decrease in the rate of large fruit. Poor commodity performance, unit benefit drops significantly

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  • Water chestnut stem tip tissue culture method
  • Water chestnut stem tip tissue culture method
  • Water chestnut stem tip tissue culture method

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Embodiment Construction

[0020] In order to make the object, technical solution and advantages of the present invention clearer, the present invention is further described in detail.

[0021] The invention provides a method for culturing water chestnut stem tip tissue, comprising the following steps:

[0022] S1 prepares the culture medium mother liquor, which is prepared from macroelements, trace elements, chelated iron, organic matter, sucrose, solidified matter, and water, and the macronutrients are selected from NH 4 NO 3 、KNO 3 , MgSO 4 ·7H 2 O. KH 2 PO 4 , CaCl 2 2H 2 O is prepared according to the concentration of 1650mg / L, 1900mg / L, 370mg / L, 170mg / L, 440mg / L respectively; 4 4H 2 O, ZnSO 4 7H 2 O, H 3 BO 3 , KI, NaMoO 4 2H 2 O, CuSO 4 ·H 2 O, CoCl 2 ·6H 2 O is prepared by the concentration of 22.3mg / L, 8.6mg / L, 6.2mg / L, 0.83mg / L, 0.25mg / L, 0.025mg / L, 0.025mg / L respectively; 2 -EDTA, FeSO 4 ·7H 2 O is formulated according to the concentration of 37.3mg / , 27.8mg / ; the organi...

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Abstract

A water chestnut stem tip tissue culture method comprises the following steps that S1, preparing culture medium mother liquor: the culture medium mother liquor is prepared from macroelements, microelements, chelated iron, organic matter, cane sugar, condensate and water; S2, preparing a plant growth regulator: the plant growth regulator is prepared from cytokinin, auxin and a plant retardant; S3, preparing a culture medium: the culture medium is prepared from water at 50-60 DEG C, carrageenan, the mother liquor and sugar; S4, selecting and disinfecting water chestnut culture buds; and S5, performing water chestnut culture bud culture and selection and seedling hardening to obtain water chestnut stem tips. According to the culture method, the variety characteristics of water chestnuts can be well kept, and the excellent advantages of the new variety are exerted.

Description

【Technical field】 [0001] The invention relates to a method for culturing stem tip tissue, in particular to a method for culturing water chestnut stem tip tissue. 【Background technique】 [0002] Water chestnut (scientific name: Heleocharis dulcis (Burm.f.) Trin.), also known as horseshoe, water chestnut, black taro, bodhisattva, etc., belongs to the monocot Cyperaceae, and is a perennial herbaceous plant. Water chestnuts are one of the famous and high-quality agricultural products in Guangxi, and also one of the traditional important foreign exchange earning products in my country. In the past ten years, my country's water chestnut industry has developed rapidly. However, for a long time, farmers kept and propagated the bulbs, resulting in serious species degradation, serious damage from diseases and insect pests, a substantial increase in control costs, a significant decrease in yield, and a decrease in the rate of large fruit. The commodity is poor, and the unit benefit dro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 江文高美萍黄诚梅蒋慧萍方彦蓉黄诗宇
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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