Ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components

A bioactive ingredient and nanoparticle technology, which is applied in the field of ultrasonic treatment technology to prepare composite nanoparticles, can solve the problems of large amount of ethanol added, increased preparation cost, unfavorable freeze-drying, etc. good stability

Active Publication Date: 2021-08-10
江苏南翔农业发展科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chen et al {Chen H, YaoY. Phytoglycogen improves the water solubility and Caco-2 monolayer permeation of quercetin[J]. Food Chemistry, 2017, 221(APR.15): 248-257.} Using phytoglycogen to embed quercetin , to construct a nano-delivery system, the solubility of quercetin is improved, but the addition of ethanol in this method is relatively large (25%), which increases the preparation cost and is not conducive to subsequent freeze-drying
Zhang et al.{Zhang Y, Yang Y, Tang K, et al.Physicochemical characterization and antioxidant activity of quercetin-loaded chitosan nanoparticles[J].Journal of Applied Polymer Science,2010,107(2):891-897.}through chitosan Ionic gelation of sugar and tripolyphosphate prepared quercetin-loaded nanoparticles, but the antioxidant activity was not high, and its DPPH free radical scavenging rate was about 50%

Method used

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  • Ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components
  • Ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components
  • Ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components

Examples

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Embodiment 1

[0040] Example 1: Optimization of casein phosphopeptide concentration in the preparation of quercetin-loaded casein phosphopeptide-chitosan composite nanoparticles

[0041] (1) dissolving the casein phosphopeptide in distilled water so that the concentrations of the casein phosphopeptide are (1.0, 1.5, 2.0, 2.5, 3.0) mg / ml respectively, and adjusting the pH of the solution to 11;

[0042] (2) Chitosan is dissolved in 1% glacial acetic acid solution, and magnetically stirred until completely dissolved;

[0043] (3) dissolving quercetin in absolute ethanol to prepare quercetin stock solution;

[0044] (4) Stir the casein phosphopeptide solution in step (1) at a constant speed, and slowly add the quercetin stock solution in step (3) according to the mass ratio of quercetin to casein phosphopeptide is 1:15;

[0045] (5) Under the condition of stirring at room temperature, according to the mass ratio of casein phosphopeptide and chitosan as 1:1, an equal volume of step (2) chitosa...

Embodiment 2

[0050] Example 2: Optimization of the mass ratio of casein phosphopeptide to chitosan in the preparation of quercetin-loaded casein phosphopeptide-chitosan composite nanoparticles

[0051] (1) dissolving the casein phosphopeptide in distilled water so that the concentration of the casein phosphopeptide is 1.5 mg / ml, and adjusting the pH of the solution to be 11;

[0052] (2) Chitosan is dissolved in 1% glacial acetic acid solution, and magnetically stirred until completely dissolved;

[0053] (3) dissolving quercetin in absolute ethanol to prepare quercetin stock solution;

[0054] (4) Stir the casein phosphopeptide solution in step (1) at a constant speed, and slowly add the quercetin stock solution in step (3) according to the mass ratio of quercetin to casein phosphopeptide is 1:15;

[0055] (5) Under the condition of stirring at room temperature, according to the mass ratio of casein phosphopeptide and chitosan (1:0, 1:3, 1:2, 1:1, 2:1, 3:1), An equal volume of step (2) ...

Embodiment 3

[0060] Example 3: Optimization of the mass ratio of quercetin to casein phosphopeptide in the preparation of quercetin-loaded casein phosphopeptide-chitosan composite nanoparticles

[0061] (1) dissolving the casein phosphopeptide in distilled water so that the concentration of the casein phosphopeptide is 1.5 mg / ml, and adjusting the pH of the solution to be 11;

[0062] (2) chitosan is dissolved in 1% glacial acetic acid solution, and magnetically stirred until completely dissolved;

[0063] (3) dissolving quercetin in absolute ethanol to prepare quercetin stock solution;

[0064] (4) The casein phosphopeptide solution in step (1) was stirred at a constant speed, according to the mass ratio of quercetin and casein phosphopeptide (1:5, 1:10, 1:12.5, 1:15, 1:20), slowly add step (3) quercetin stock solution;

[0065](5) Under the condition of stirring at room temperature, according to the mass ratio of casein phosphopeptide and chitosan as 1:1, an equal volume of step (2) ch...

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Abstract

The invention discloses an ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components, and relates to the technical field of food embedding. The preparation method comprises the following steps: under magnetic stirring, dropwise adding a chitosan solution containing 1% of glacial acetic acid into a casein phosphopeptide solution added with a quercetin stock solution in an equal volume manner, adjusting the pH value of the system, carrying out ultrasonic treatment on the mixed solution, and after ultrasonic treatment is finished, carrying out freeze drying to obtain the casein phosphopeptide-chitosan nanoparticles loaded with quercetin. In the process of embedding the quercetin by utilizing the electrostatic interaction between the casein phosphopeptides and the chitosan, a multi-mode ultrasonic treatment technology is used, the cross-linking of polypeptide and polysaccharide is promoted through the physical force of ultrasonic waves, and a basis is provided for embedding more bioactive components in the compound. The product obtained by the invention is high in quercetin embedding rate, good in solubility in water, excellent in light and heat stability and relatively strong in oxidation resistance, and the bioavailability of the quercetin in the stomach and intestine is greatly improved.

Description

technical field [0001] The invention relates to the technical field of food embedding, in particular to a method for loading bioactive substance quercetin with casein phosphopeptide and chitosan as raw materials, and adopting ultrasonic treatment technology to prepare composite nanoparticles. Background technique [0002] Quercetin is a flavonoid, which is a biologically active component. It has the advantages of anti-oxidation, anti-tumor, and lowering blood pressure and blood lipids. However, quercetin has disadvantages such as low water solubility, poor stability, and low bioavailability, which seriously limit its application in the fields of food and medicine. Studies have shown that the assembly of active substances through nano-embedding to form composite nanoparticles can not only provide essential nutrients for the human body, but also improve the water solubility, stability and bioavailability of hydrophobic substances. [0003] Natural biological macromolecules (p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/352A61K47/36A61K47/42A61P3/06A61P9/12A61P35/00A61P39/06A61K8/49A61K8/64A61K8/73A61Q19/08A23L33/105A23P10/30
CPCA61K9/5169A61K9/5161A61K9/5192A61K31/352A61P39/06A61P35/00A61P9/12A61P3/06A61K8/498A61K8/736A61K8/64A61Q19/08A23L33/105A23P10/30A61K2800/56A61K2800/82A23V2002/00A23V2200/302A23V2200/308A23V2200/3262A23V2200/326A23L5/32A23L33/18A23L29/275A23P10/40A61K47/36A61K47/42
Inventor 任晓锋孙心如梁秋芳马海乐刘雪琼汤佳琳周成伟
Owner 江苏南翔农业发展科技有限公司
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