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Recombinant polypeptides and vaccines for preventing and treating Eimeria spp.

A recombinant polypeptide and vaccine technology, applied in vaccines, recombinant DNA technology, and microbial-based methods, can solve problems such as no systematic understanding of coccidia and host cells, difficulties in the development of new anticoccidial drugs and molecular vaccines, and achieve control Chicken coccidiosis and the effect of reducing the amount of use

Active Publication Date: 2022-04-12
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no systematic understanding of the detailed interaction mechanism between coccidia and host cells, resulting in great difficulties in the development of new anticoccidial drugs and molecular vaccines

Method used

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  • Recombinant polypeptides and vaccines for preventing and treating Eimeria spp.
  • Recombinant polypeptides and vaccines for preventing and treating Eimeria spp.
  • Recombinant polypeptides and vaccines for preventing and treating Eimeria spp.

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preparation example Construction

[0032] The present invention also relates to the preparation method of the above-mentioned recombinant polypeptide, comprising:

[0033] Cultivate the above-mentioned host cells under appropriate conditions, collect the culture medium and / or the lysate of the host cells, separate and purify to obtain the recombinant polypeptide.

[0034] The present invention also relates to a vaccine comprising a recombinant polypeptide as described above, a nucleic acid as described above or a vector as described above.

[0035] In some embodiments, the vaccine further comprises an adjuvant and / or a pharmaceutically acceptable carrier.

[0036] As used herein, an "adjuvant" is a substance that facilitates or amplifies a cascade of immunological events that ultimately leads to a better immune response, ie, an integrated body response against an antigen. An adjuvant is not usually required for an immune response to occur, but facilitates or amplifies it.

[0037]Adjuvants can also be aluminu...

Embodiment

[0057] 1 Preparation of EA-FLTI coding gene

[0058] 1.1 Optimization of coding genes

[0059] According to the bioinformatics analysis of the related genes encoded by the Eimeria heaperia micronematin and the rod neck protein obtained by the applicant team, the rod neck protein 2 (109 amino acids) and the rod neck protein 1 were screened. (202 amino acids) and 2 (206 amino acids) and as candidate tandem polypeptides, the amino acid sequence is as shown in SEQ ID NO: 3, codon optimization is carried out for different hosts, and the prokaryotic expression coding gene sequences are respectively obtained, such as SEQ ID NO: 1, and the eukaryotic expression coding gene sequence, as shown in SEQ ID NO:2.

[0060] 1.2 Synthesis of coding genes

[0061] The EA-FLTI prokaryotic coding gene (SEQ ID NO: 1) and eukaryotic coding gene (SEQ ID NO: 2) were prepared using the whole gene synthesis technology, and the T vector plasmids (pMD18-T-EA-FLTI1 and pMD18- T-EA-FLTI2).

[0062] 2 P...

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Abstract

The invention relates to the field of veterinary biological products, in particular to a recombinant polypeptide and a vaccine used for preventing and treating Eimeria spp. By immunizing the Eimeria hemophilia recombinant polypeptide vaccine EA-FLTI protein, it can effectively control the infection of Eimeria hemophilia in chickens, greatly reduce the use of anticoccidiostats in chicken farms, and effectively control chicken coccidiosis .

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a recombinant polypeptide and a vaccine used for preventing and treating Eimeria spp. Background technique [0002] Eimeria acervulina (E.acervulina) is an obligate intracellular parasitic apicomplexan protozoa, which can cause serious harm to chicken coccidiosis in intensive chicken farming. In the absence of preventive measures or failure of prevention (such as drug ineffective due to drug resistance problems), the chicken morbidity rate can reach 30% to 100%, and the lethal rate can be as high as 80%. The global economic loss caused by chicken coccidiosis exceeds more than 3 billion US dollars every year. At present, the prevention and control of coccidiosis in chickens is still mainly carried out by the technical methods of "adding various anticoccidial drugs to the feed for drug prevention and control" and "live oocyst vaccine for vaccine prevention and control...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/455C12N15/30C12N15/70C12N1/21A61K39/012A61P33/02C12R1/19
CPCC07K14/455C12N15/70A61K39/012A61P33/02C12N2800/22A61K2039/53
Inventor 孙铭飞戚南山廖申权吕敏娜吴彩艳李娟蔡海明林栩慧胡俊菁于林增肖文婉张小慧张健騑
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI