Automatic leveling and automatic focusing method for bone marrow smear scanning

Abstract

The invention relates to an automatic leveling method for bone marrow smear scanning, which comprises the following steps: (1) a mechanical arm grabs a bone marrow smear from a slide box, the bone marrow smear is moved below a laser range finder, and a square scanning area is selected on the bone marrow smear as a frame selection area; (2) the laser range finder firstly performs scanning range finding on the boundary of the left side of the frame selection area of the bone marrow smear and then performs scanning range finding on the boundary of the right side of the frame selection area of the bone marrow smear to form a data set; (3) the mechanical arm adjusts the bone marrow smear according to the difference value, measured in the step (2), of the left boundary and the right boundary of the frame selection area, the left boundary and the right boundary of the frame selection area are adjusted to the same height, and the distance value under the condition is measured. The bone marrow smear is leveled and then focused, accurate focusing of the bone marrow smear is achieved, the method is easy to operate, the distance serves as the unique standard of focusing, focusing in the bone marrow smear shooting process can be achieved, and the method is not affected by uneven thickness of the bone marrow smear and different cell sizes.

Description

technical field [0001] The invention belongs to the field of artificial intelligence medical image processing, in particular to an automatic focus method for bone marrow smear scanning. Background technique [0002] At present, the scanning equipment for bone marrow smear generally places the bone marrow smear on the stage, and the microscope lens is installed on the mechanical device. The up and down movement of the mechanical device drives the microscope lens to move to achieve the focus on the bone marrow smear. There are the following technical difficulties: the depth of field of the 100-power lens of the microscope is small, and when making bone marrow smears, the uneven thickness of the smears, the difference in cell size, and the existence of impurities on the smears often affect the hardware equipment and algorithms. The degree of precision required is very high. [0003] At present, there are mainly two focusing methods for smears. One is the contrast focusing meth...

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly two focusing methods for smears. One is the contrast focusing method, which achieves focusing by calculating the amount of contrast between adjacent images. Minute

The other is fast focusing, mainly through rough adjustment first, and then fine adjustment to complete the focus. This method requires a large amount of algorithms and the process is relatively complicated.

In addition, the above two focusing methods may cause damage to the bone marrow smear during the focusing process

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