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Human intestinal cancer primary cell and culture method and application thereof

A technology for primary cells and intestinal cancer cells, which can be used in cell culture active agents, gastrointestinal cells, tumors/cancer cells, etc. It can solve problems such as the research of unfavorable pathogenesis and treatment plan, and the easy variation of biological characteristics.

Inactive Publication Date: 2021-08-20
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after long-term culture, the biological characteristics of the cell line are prone to variation, which is not conducive to the research of pathogenesis and treatment plan.

Method used

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  • Human intestinal cancer primary cell and culture method and application thereof
  • Human intestinal cancer primary cell and culture method and application thereof
  • Human intestinal cancer primary cell and culture method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0044] The embodiment of the present invention provides a primary human intestinal cancer cell named as human intestinal cancer cell HCOC-883, which is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No. 21096.

[0045] The human intestinal cancer cell HCOC-883 was first isolated and cultured from Chinese intestinal cancer tissue, and the cells did not introduce any foreign genes.

[0046] Next, each biological characteristic of human intestinal cancer cell HCOC-883 will be described.

[0047] 1.1 Cell morphology observation

[0048] Observe the human intestinal cancer cell HCOC-883 under an inverted microscope, the epithelioid cell shape, the cancer cell proliferation is fast, the cell size and shape are different, the cell boundaries are clear, irregular or polygonal, losing polarity, disordered, no Contact-inhibited, paving-stone-like growth with morphological features of malignant ...

Embodiment 2

[0152] This example is used to illustrate the culture method of human intestinal cancer cell HCOC-883, including the following steps 1-4.

[0153] In step 1, the collected fresh human intestinal cancer tissue is preserved with a sample preservation solution. Wherein, the sample preservation solution includes fetal bovine serum, antibiotics and DF medium.

[0154] In step 2, the colorectal cancer tissue preserved in step 1 is washed with a washing solution. Wherein, the cleaning solution includes physiological saline containing the antibiotic.

[0155] In step 3, the intestinal cancer tissue cleaned and treated in step 2 is taken, and the serum-containing medium is used for primary isolation and culture. Wherein, the serum-containing medium includes fetal bovine serum, the antibiotic and DF medium.

[0156] In step 4, the primary isolated and cultured cells in step 3 are purified and subcultured in a serum-free medium to obtain primary human intestinal cancer cells. Wherein...

Embodiment 3

[0204] This example is used to illustrate the use of human intestinal cancer cell HCOC-883 for non-disease diagnosis and treatment purposes.

[0205] The application of the human intestinal cancer cell HCOC-883 provided in the embodiments of the present invention can be specifically used for drug sensitivity research and detection, establishment of intestinal cancer animal models, development of intestinal cancer inhibitory drugs, and in vitro research of intestinal cancer, etc.

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Abstract

The invention provides a human intestinal cancer primary cell and a culture method and application thereof. The human intestinal cancer primary cell is named as human intestinal cancer cells HCOC-883 and preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.21096.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to human intestinal cancer primary cells and their culture method and application. Background technique [0002] Colon cancer is one of the common malignant tumors in digestive system tumors, which seriously endangers human health. At present, in order to study its pathogenesis and treatment plan, most of the commercialized cell lines are used as materials. However, after long-term culture, the biological characteristics of cell lines are prone to variation, which is not conducive to the study of pathogenesis and treatment. [0003] Primary tumor cell culture refers to the process of surgically resecting tumor tissue from a tumor patient, obtaining tumor cells, and culturing them in vitro for a short period of time. Different from cell lines, the biological characteristics of primary cultured tumor cells have not changed much because the tissue has just been isolated from the body, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027
CPCC12N5/0693C12N5/0679G01N33/5011A01K67/0271C12N2500/90C12N2500/32C12N2500/25C12N2501/11C12N2500/38C12N2503/02G01N2500/10A01K2207/12A01K2227/105A01K2267/03
Inventor 李春凤智慧芳倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD