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Valuing method of leucine-rich alpha-2 glycoprotein 1 standard substance

A standard substance, leucine-rich technology, applied in the field of protein analysis and detection, to achieve the effect of ensuring accuracy, reducing usage, and improving sensitivity

Pending Publication Date: 2021-08-20
HANGZHOU GUANGKEANDE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the actual detection work, the immunoassay method is used to measure LRG1, and the LRG1 protein standard substance is required, but there is no report on the traceability analysis of the LRG1 standard substance.

Method used

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  • Valuing method of leucine-rich alpha-2 glycoprotein 1 standard substance
  • Valuing method of leucine-rich alpha-2 glycoprotein 1 standard substance
  • Valuing method of leucine-rich alpha-2 glycoprotein 1 standard substance

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Experimental program
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Embodiment 1

[0061] A method for determining the value of a leucine-rich alpha-2 glycoprotein 1 standard substance, comprising the following steps:

[0062] Step 1: Screen the characteristic peptides of LRG1 and stable isotope-labeled internal standard peptides;

[0063] Step 2: Optimizing the analysis conditions of nanoliter liquid chromatography-mass spectrometry for characteristic peptides and internal standard peptides;

[0064] Step 3: Mix the characteristic peptides at different concentrations and the internal standard peptide at a fixed concentration in equal volumes, analyze using the optimized nanoliter liquid chromatography-mass spectrometry method in step 2, and establish a standard curve;

[0065] Step 4: Enzymolyze the LRG1 standard substance, add the internal standard peptide into the reaction solution after enzymolysis, and analyze it with the optimized nanoliter liquid chromatography-mass spectrometry method in step 2, and combine the selected characteristic peptide with th...

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Abstract

The invention discloses a valuing method of a leucine-rich alpha-2 glycoprotein 1 standard substance. The valuing method of the leucine-rich alpha-2 glycoprotein 1 (LRG1) standard substance has the beneficial effects that the content of the LRG1 standard substance is measured by using an isotope dilution mass spectrometry, so that the blank in the prior art is filled, the accuracy, reliability and traceability of a valuing result are ensured, and the method has important significance for establishing a quality control and evaluation system of the LRG1 standard substance.

Description

technical field [0001] The invention relates to a protein analysis and detection technology, in particular to a method for determining the value of a leucine-rich alpha-2 glycoprotein 1 (LRG1) standard substance. Background technique [0002] With the vigorous development of the field of life sciences, protein analysis and detection technology is widely used in clinical medical testing, biomedical analysis and other fields. In clinical medical testing, many disease markers such as alpha-fetoprotein, carcinoembryonic antigen, and C-reactive protein are proteins, so the accuracy of the quantitative results of these protein markers has a significant impact on the diagnosis and prognosis of diseases. [0003] At present, for the quantitative analysis of target protein markers, immunological analysis methods based on antigen-antibody reactions are mostly used. The immunological analysis method has fast detection speed, high sensitivity and good specificity, but its accuracy and ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 高俊莉宋林珍江南王洪高俊顺
Owner HANGZHOU GUANGKEANDE BIOTECHNOLOGY CO LTD