Fusion protein and application thereof

A fusion protein and protein technology, applied in fusion protein and its application field, can solve problems affecting protein-DNA interaction, gene silencing, etc.
CN113307878APending Publication Date: 2021-08-27SHANDONG SHUNFENG BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG SHUNFENG BIOTECH CO LTD
Publication Date
2021-08-27

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Abstract

The invention provides a fusion protein and an application thereof. Specifically, the invention provides a fusion protein, which comprises or consists of the following components: (1) a positioning functional element D1 with functions of targeting and combining DNA; and (2) a demethylated functional element D2 having a function of converting methylated nucleotides into non-methylated nucleotides. The demethylation method provided by the invention has accurate and efficient demethylation modification efficiency in plants, and has important scientific values for researching plant epigenetics and regulating plant traits through demethylation.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a fusion protein and its application. Background technique

[0002] DNA methylation is a form of chemical modification of DNA, which can change genetic expression without changing the DNA sequence, and is a common modification method in eukaryotic cells. The establishment of DNA methylation is formed by the reaction catalyzed by DNA methyltransferase with S-adenosylmethionine (SAM) as the methyl donor.

[0003] There are many ways of methylation modification. The bases at the modified site can be the N-6 position of adenine (6mA), the N-4 position of cytosine, the N-7 position of guanine (7mG) and cytosine The C-5 position (5mC). They are respectively catalyzed by different DNA methylases. However, the most well-studied and most common is the methylation of 5mC, the C-5 position of cytosine.

[0004] Methylated DNA can undergo demethylation. There are two mechanisms ...

Claims

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