Closed bipolar electrode array and application thereof
A bipolar electrode and closed technology, which is applied in the direction of electrochemical variables of materials, material analysis by electromagnetic means, and analysis by making materials undergo chemical reactions, can solve the problem of high quantum yield, insufficient current, and inability to achieve accurate detection. Markers and other problems, to achieve the effect of large application potential, rich color changes, and not easy to misdiagnose
Active Publication Date: 2021-08-31
NANJING UNIV
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Problems solved by technology
Although the BPE-multicolor ECL method has the advantages of intuition, low cost and high throughput, it can only roughly determine the concentration range of biomarkers at present, and cannot achieve accurate detection of markers. The limited choice of ECL luminescence reagents with complete separation of view, potential, and wavelength and insufficient current flow through BPE severely limit the sensitivity of the BPE-multicolor ECL method
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Embodiment 1
[0042] Example 1 Multi-color electrochemiluminescence technology combined detection of multiple prostate cancer stage indicator factors
[0043] Fabrication of the BPE array: Use drawing software to draw the required BPE array pattern, and then transfer the pattern to the indium tin oxide conductive glass (ITO glass) by screen printing technology and mixed etching method. Then, the polydimethylsiloxane (PDMS) with the detection cell is bonded to the above-mentioned conductive glass with the electrode pattern, and the detection cell is aligned with the detection electrode. A closed BPE made of ITO glass and a PDMS mold is used, and the two ends of the closed BPE are electrically connected to the anode liquid storage pool and the cathode liquid storage pool respectively.
[0044] Protection of the cathode end of the BPE array: all reservoirs are filled with 60 μL of HAuCl 4 (2.4mM), and then apply a driving voltage (5.0V) for 300s, and finally rinse the electrode with water and...
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The invention discloses a closed bipolar electrode array and an application thereof. The closed bipolar electrode array is formed by connecting three closed bipolar electrodes in parallel, luminescent substances [Ir (ppy) 2 (acac)], [Irpic-oMe] and [Ru (bpy) 3] < 2 + > are used, when TPrA serves as a co-reactant, due to the fact that the three luminescent substances emit green light, blue light and red light respectively, the light-emitting potential of the three light-emitting substances is completely separated (the light-emitting potential of [Ir (ppy) 2 (acac)] is 0.75 V, the light-emitting potential of [Ir (pic)-oMe] is 0.95 V, and the light-emitting potential of [Ru (bpy) 3] < 2 + > is 0.87 V) so that along with the increase of the current on the bipolar electrode, the light-emitting color covers the whole visual area, and the color change is richer; three prostate cancer staging indication factor markers are combined for detection (mRNA-141, mRNA-121 and mRNA-19a), misdiagnosis is not prone to occurring, and the staging of cancer patients is rapidly judged; and the detection of the three indicator factors can be integrated on one chip so that high throughput is achieved, and the electrode array has a great application potential in the development of clinical detection equipment.
Description
technical field [0001] The invention belongs to the technical field of electrochemiluminescence biological detection, and relates to a method for joint detection of prostatic cancer staging indicator factors using a multicolor electrochemiluminescence technology combined with a bipolar electrode array, in particular to a closed bipolar electrode array and its application. Background technique [0002] Prostate cancer is one of the common malignant tumors of the male reproductive system. At present, the best clinical treatment for prostate cancer is radical surgery, and accurate and rapid preoperative staging of prostate cancer is the key to choosing treatment. Prostate cancer staging refers to the degree of development of prostate cancer in the prostate, adjacent tissues around the prostate, and distant organs. It is mainly judged by detecting prostate cancer staging indicators in blood or urine. Micro-ribonucleic acids (miRNAs for short) of prostate cancer (such as miRNA-1...
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IPC IPC(8): G01N21/76G01N27/327G01N27/48
CPCG01N21/763G01N27/3275G01N27/48
Inventor 徐静娟王银珠赵微陈洪渊
Owner NANJING UNIV