A kind of 17(15→16)-shift-rosinane type diterpene compound, preparation method, pharmaceutical composition and application
A compound, the technology of abietane, which is applied in the field of anti-tumor compounds, can solve the problems that have not been reported, achieve obvious inhibitory effect, and improve the effect of medical and economic value
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Embodiment 1
[0034] A 17(15→16)-shift-rosinane type diterpene compound with antitumor effect, the chemical name is: 11,14,16-trihydroxy-6,12-dimethoxy-17(15→16 )-shift-5,8,11,13-rosinanetetraene-3,7-dione, the structure is shown in the following formula I:
[0035]
[0036] 1. Physical and chemical properties test of the above compound Bracteatum B.
[0037] 1.1 Determination of molecular structure
[0038] The nuclear magnetic resonance apparatus used is a Bruker AVANCE III 500 nuclear magnetic resonance apparatus manufactured by Bruker Company, and the nuclear magnetic resonance uses deuterated CDCl 3 (deuterated chloroform) reagent, the hydrogen spectrum and carbon spectrum data of gained rosin alkyl diterpene compound Bracteatum B are shown in Table 1 below, and the results are as follows figure 1 , 2 shown.
[0039] The hydrogen spectrum of Table 1Bracteatum B 1 H-NMR (500MHz) and carbon spectrum 13 C-NMR (125MHz) data
[0040]
[0041] Note: Chemical shifts are represent...
Embodiment 2
[0047] The preparation method of the rosinane type diterpene compound BracteatumB in embodiment 1, comprises the following steps:
[0048] (1) Air-drying and pulverizing the branches of 9 kg of Daqing buds, adding them to ethanol with a volume fraction of 90% for extraction, the addition amount is 10 mL / g of ethanol, and the extraction temperature is 70 ° C, and the extraction is performed 3 times, 5 hours each time; filter the insolubles and combine the filtrates, then evaporate to dryness at low temperature under vacuum at 0.08MPa and a temperature of 45°C to obtain the extract;
[0049] (2) the obtained extract and water are mixed by mass ratio of 1:2 to make suspension, equal volume ethyl acetate is extracted 3 times, and the extract is merged, and the vacuum degree is 0.08MPa, and the temperature is a low temperature at 45 ℃ Evaporate to dryness under reduced pressure to obtain an ethyl acetate extract;
[0050] (3) the obtained ethyl acetate extraction phase is mixed we...
Embodiment 3
[0053] The preparation method of the rosinane type diterpene compound BracteatumB in embodiment 1, comprises the following steps:
[0054] (1) Air-dry and pulverize the branches of 9 kg of Daqing buds, add them into ethanol with a volume fraction of 80% for extraction, the addition amount is 20 mL / g of ethanol, and the extraction temperature is 65°C, and the extraction is performed 3 times. , 4 hours each time; filter the insolubles and combine the filtrates, and then evaporate to dryness at a low temperature under vacuum at 0.07MPa and a temperature of 50°C to obtain the extract;
[0055] (2) the obtained extract and water are mixed homogeneously at a mass ratio of 1:1 to make a suspension, and the equal volume of ethyl acetate is extracted 4 times, and the extracts are combined, and the vacuum degree is 0.07MPa, and the temperature is a low temperature of 50 ℃. Evaporate to dryness under reduced pressure to obtain an ethyl acetate extract;
[0056] (3) the obtained ethyl ac...
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