Fixed observation device for living imaging of animal sciatic nerve

Abstract

The invention discloses a fixed observation device for living imaging of animal sciatic nerve, and relates to the technical field of observation devices. The fixed observation device comprises a base; a fixed arm which is fixedly arranged on the base; one end of a fixed rod is arranged on one side, far away from the base, of the fixed arm; one end of an observation piece is fixedly connected with the end, away from the fixed arm, of the fixed rod, the observation piece is located at an opening of an animal incision, the observation piece is provided with a visible window, and the side, away from the fixed arm, of the observation piece is provided with a fixed hole; and a slide which is covered on the visible window. A fixed assembly used for fixing the fixed rod to the fixed arm or separating the fixed rod from the fixed arm is arranged on the side, away from the base, of the fixed arm. By the adoption of the technical scheme, a user can conveniently observe living nerves through a two-photon microscope for a long time, incision infection caused by repeated surgeries on animals is avoided, and pollution caused by multiple surgeries on the animals is reduced.

Description

technical field [0001] The invention relates to the technical field of observation devices, in particular to a fixed observation device for live imaging of animal sciatic nerves. Background technique [0002] In the experimental research in the field of neurobiology, the traditional method of in vivo imaging of animal peripheral nerves is to expose the nerves to be observed through surgery, and then use a fluorescence microscope or a two-photon microscope to perform dynamic imaging of the exposed nerves in vivo. After the imaging, the muscles and skin were sutured layer by layer, and the skin and muscles were incised for the next observation to expose the original nerves and repeated. However, this process cannot last for a long time. Prolonged anesthesia and repeated operations will not only harm the animal's systemic function, but also increase the risk of infection, increase the inflammatory response, cause tissue damage, and cause more pain for the animal. Not conducive...

AI Technical Summary

Problems solved by technology

However, this process cannot last for a long time. Prolonged anesthesia and repeated operations will not only harm the animal's systemic function, but also increase the risk of infection, increase the inflammatory response, cause tissue damage, and cause more pain to the animal. Not conducive to further observation and experimental research

Moreover, there is currently no uniform standard for surgical fixation and postoperative observation of external fixators, resulting in complicated surgical operations and difficulties in postoperative imaging and fixation.

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