A kind of Dendrobium officinale leaf polysaccharide and its preparation and application

A technology of Dendrobium officinale and polysaccharides, applied in the field of natural polymers, can solve the problems of inability to accurately verify the activity and function of Dendrobium polysaccharides, and achieve the effect of improving immunity, restoring normal health, and simple process

Active Publication Date: 2022-07-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention degreases petroleum ether, removes monosaccharides with ethanol, and obtains polysaccharides after repeated decoction and alcohol precipitation. This invention verifies that Dendrobium polysaccharides have certain effects on intestinal flora during in vitro fermentation, but it cannot accurately verify the effect of Dendrobium polysaccharides on organisms. active function in
[0010] Therefore, both the preparation method of Dendrobium officinale polysaccharides and the activity and function of Dendrobium leaf polysaccharides in organisms need further in-depth research.

Method used

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  • A kind of Dendrobium officinale leaf polysaccharide and its preparation and application
  • A kind of Dendrobium officinale leaf polysaccharide and its preparation and application
  • A kind of Dendrobium officinale leaf polysaccharide and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) 100g of Dendrobium officinale leaves are washed, dried at 65°C, pulverized and crossed through a 20-mesh sieve;

[0053] (2) soaking the above materials in 70% ethanol solution for 8h, 80% ethanol for 8h, and 90% ethanol for 8h to remove impurities;

[0054] (3) The ethanol in the soaked samples was dried and removed, and 30 times the volume of water was added for extraction at 80° C. for 3 hours, and the extracts were combined after repeating 3 times;

[0055] (4) Add protease to the above extract, carry out the enzymatic hydrolysis reaction at 35°C for 3h, after completion, heat the solution to 95°C for 20min inactivation, and centrifuge to obtain the supernatant;

[0056] (5) adding 0.1% activated carbon to the above-mentioned supernatant for decolorization, and centrifuging to obtain the supernatant;

[0057] (6) the above-mentioned supernatant was concentrated, and 80% ethanol was added to stand for 12h, and the precipitate was obtained after centrifugation; ...

Embodiment 2

[0060] (1) 100g of Dendrobium officinale leaves are washed, dried at 85°C, pulverized and crossed a 30-mesh sieve;

[0061] (2) soaking the above materials in 85% ethanol solution for 12h and 95% ethanol for 12h successively to remove impurities;

[0062] (3) The ethanol in the above soaked samples was dried and removed, and 10 times the volume of water was added at 95°C for extraction for 2 hours, and the extracts were combined after repeating 5 times;

[0063] (4) Add protease to the above extract, carry out the enzymolysis reaction at 37°C for 1h, after completion, heat the solution to 100°C for 30min for inactivation, and centrifuge to obtain the supernatant;

[0064] (5) adding 0.2% activated carbon to the above-mentioned supernatant for decolorization, and centrifuging to obtain the supernatant;

[0065] (6) Concentrate the above-mentioned supernatant, add 95% ethanol and let stand for 12h, and obtain a precipitate after centrifugation;

[0066] (7) Dissolving the abov...

Embodiment 3

[0084] (1) 100g of Dendrobium officinale leaves are washed, dried at 50°C, pulverized and crossed through a 60-mesh sieve;

[0085] (2) soak the above materials in 60% ethanol solution for 6 hours, 70% ethanol for 6 hours, 80% ethanol for 6 hours, and 90% ethanol for 6 hours to remove impurities;

[0086] (3) The ethanol in the above soaked samples was dried and removed, and 25 times the volume of water was added at 75° C. for extraction for 2 hours, and the extracts were combined after repeating 3 times;

[0087] (4) Add protease to the above extract, carry out enzymolysis reaction at 40°C for 2h, after completion, heat the solution to 90°C for 15min for inactivation, and centrifuge to obtain supernatant;

[0088] (5) adding 0.15% activated carbon to the above-mentioned supernatant for decolorization, and centrifuging to obtain the supernatant;

[0089] (6) Concentrate the above-mentioned supernatant, add 85% ethanol and let stand for 12h, and obtain a precipitate after cent...

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Abstract

The invention discloses a Dendrobium officinale leaf polysaccharide and its preparation and application. The preparation method is carried out in sequence according to the following steps: (1) washing, drying and pulverizing the Dendrobium officinale leaves; (2) soaking the processed material in an aqueous ethanol solution with a volume concentration of 60%-95% to extract to Remove impurities, wherein the extraction times are 2-6 times, and the concentration of the aqueous ethanolic solution used in the latter extraction is higher than the concentration of the aqueous ethanolic solution used in the previous extraction, so as to obtain the soaked sample; (3) the soaked sample is extracted with water to obtain a water extract; ( 4) enzymolysis with protease; (5) decolorization with activated carbon; (6) concentrating the supernatant, adding an aqueous ethanol solution and leaving it to stand overnight, and centrifuging to obtain a precipitate; (7) dissolving the precipitate in distilled water, and freeze-drying to obtain Dendrobium officinale leaves polysaccharides. The dendrobium leaf polysaccharide prepared by the invention has the effect of improving immune deficiency and restoring normal health, and can be used for preparing food or health care product with the effect of improving immune deficiency.

Description

(1) Technical field [0001] The invention relates to the technical field of natural macromolecules, in particular to a Dendrobium officinale leaf polysaccharide and its preparation and application in the preparation of foods or health-care products with the effect of improving immune deficiency. [0002] (2) Technical background [0003] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is a perennial epiphytic herb of the orchid family. It is sweet in taste and slightly cold in nature. In the modern food and pharmaceutical industry, Dendrobium has been used in many diseases with its good therapeutic effect. However, in traditional cognition, only Dendrobium stems have application value, and other parts such as flowers and leaves are often directly discarded or simply processed, resulting in a huge waste of resources. Studies in recent years have found that some active ingredients in dendrobium leaves can also treat some diseases. Therefore, how to e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/125A61K31/715A61K36/8984A61P37/04A61K127/00
CPCC08B37/0003A23L33/125A61K31/715A61K36/8984A61P37/04A23V2002/00A23V2200/324A23V2250/51
Inventor 邵平谢华凌陈碧莲陆柏益方静宇
Owner ZHEJIANG UNIV OF TECH
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