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Organoid forming chip based on microfluidic technology, and working method of organ-like forming chip

A microfluidic technology and organoid technology, applied in the field of biological microfluidics, can solve the problems such as the inability to strictly guarantee the uniform size of the organoids and the uneven sedimentation.

Active Publication Date: 2021-09-10
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problem that the microwell arrays in the existing microfluidic forming chips are arranged horizontally, when the cell tissue blocks of different sizes settle under the action of gravity, the problem of uneven settlement is still quite obvious, and the organoids formed by culture cannot be strictly guaranteed. defects of uniform size, the present invention provides an organoid forming chip based on microfluidic technology and its working method

Method used

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  • Organoid forming chip based on microfluidic technology, and working method of organ-like forming chip
  • Organoid forming chip based on microfluidic technology, and working method of organ-like forming chip
  • Organoid forming chip based on microfluidic technology, and working method of organ-like forming chip

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Effect test

Embodiment 1

[0040] Such as Figure 1 to Figure 3 As shown, an organoid forming chip based on microfluidic technology, which includes a bottom flow channel 1 and a top cover plate 2 covered on the top of the bottom flow channel 1, and the bottom flow channel 1 is sequentially provided with A liquid flow inlet 3, a culture pool array 4 communicated with the liquid flow inlet 3, and a culture liquid outlet 5 for discharging the liquid flow passing through the culture pool array 4, the culture pool array 4 is connected to the bottom flow The bottom surface of the channel 1 is inclined at a certain angle, and the end of the culture pool array 4 close to the culture solution outlet 5 is higher than the end of the culture pool array 4 close to the liquid inflow inlet 3, and the culture pool array 4 is inclined. The micro-tissue blocks in the micro-tissue block suspension can be blocked from flowing along the flow direction of the micro-tissue block suspension to fill up each culture pool 41 in t...

Embodiment 2

[0052] A working method for forming an organoid chip based on microfluidic technology, which includes the following steps:

[0053] S1. After the primary tissue is cut and enzymatically treated in the laboratory, a suspension of microtissue blocks is formed;

[0054] S2. The injection liquid of the micro-tissue block suspension flows into the inlet 3, and then the micro-tissue block suspension passes through the primary screening structure 8 and the secondary screening structure 9 sequentially under the driving force, and moves to the culture pool array 4, wherein, the micro-tissue block suspension cannot pass through The microtissue fragments of the primary screening structure (8) are drained through the flow channel to the primary screening liquid outlet (10) for collection, and the microtissue fragments that can pass through the secondary screening structure (9) are drained through the flow channel to the secondary screening liquid outlet (11) to collect;

[0055] S3. When...

Embodiment 3

[0065] This embodiment is similar to Embodiment 2, and the difference is that the following steps are also included between the step S3 and the step S4:

[0066] S5. After digesting the oversized tissue collected at the outlet 10 of the primary screening fluid, mix it again with the suspension of the microtissue block collected at the outlet 5 of the culture fluid, and then mix it with the injection fluid inlet 3, and repeat steps S2 to S3, thus The process is repeated several times, so as to collect the same amount of microtissues in each culture pool 41 .

[0067] Other steps in this embodiment are the same as in Embodiment 2.

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Abstract

The invention provides an organoid forming chip based on a microfluidic technology. The organoid forming chip based on the microfluidic technology comprises a bottom-layer flow channel and a top-layer cover plate covering the top of the bottom-layer flow channel, wherein the bottom-layer flow channel is provided with a liquid flow inlet, a culture pond array and a culture solution outlet in sequence; the culture pond array communicates with the liquid flow inlet; the culture solution outlet is used for discharging liquid flow which passes through the culture pond array; the culture pond array and the bottom surface of the bottom-layer flow channel are obliquely arranged at a certain included angle; and one end, which is close to the culture solution outlet, of the culture pond array is higher than one end, which is close to the liquid flow inlet, of the culture pond array. The invention further provides a working method of the organoid forming chip based on the microfluidic technology. According to the invention, the consistency of the initial number of cells retained in each culture pond of the culture pool pond in the organoid forming chip can be improved, and the formed organoid has good uniformity.

Description

technical field [0001] The invention relates to the technical field of biological microfluidics, and more specifically, to an organoid forming chip based on microfluidics technology and a working method thereof. Background technique [0002] Organoids are a model based on a 3D in vitro cell culture system that is highly similar to the source tissue or organ in vivo. These 3D in vitro culture systems can replicate the complex spatial morphology of differentiated tissues, and can show the interaction and spatial position morphology between cells and between cells and their surrounding matrix. Its characteristics must contain more than one cell type identical to the source organ; it should exhibit some functions unique to the source organ; the organization of cells should be similar to that of the source organ. Organoid technology has applications in a broad range of fields, including developmental biology, disease pathology, cell biology, mechanisms of regeneration, precision...

Claims

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Application Information

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IPC IPC(8): C12M3/00B01L3/00
CPCC12M21/08C12M23/16C12M23/20C12M23/38C12M25/14B01L3/5027B01L3/502753Y02P60/21
Inventor 刘利彪邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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