Primer composition, product and method for detecting PKD1 variant single sperm

A primer composition and single sperm technology, applied in biochemical equipment and methods, recombinant DNA technology, microbe determination/testing, etc., can solve problems such as difficult collection of family member samples, insufficient female eggs, etc.

Pending Publication Date: 2021-09-14
PEKING JABREHOO MED TECH CO LTD
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, it is difficult for the present invention to collect complete family member samples; the high pathogenicity risk haplotype carried by the man is derived from the new variation of the man's chromosome, rather than being inherited from the man's parents, which increases the detection At the same time, the age of couples undergoing PGT-M is getting older, and the situation of women's insufficient eggs is also a problem that needs to be considered

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer composition, product and method for detecting PKD1 variant single sperm
  • Primer composition, product and method for detecting PKD1 variant single sperm
  • Primer composition, product and method for detecting PKD1 variant single sperm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Primer composition

[0048] There are 216 pairs of primers (as shown in Table 1), which can specifically amplify the PKD1 gene and the region where closely linked single nucleotide polymorphism (SNP) sites are located within 2 Mb upstream and downstream. The characteristics of the primer are: unique sequence on the target chromosome; position-specific oligonucleotides have the same annealing temperature.

[0049] Table 1: Primers for detection of PKD1 variant single sperm

[0050]

[0051]

[0052]

[0053]

[0054]

[0055]

[0056]

[0057]

[0058]

[0059]

[0060]

[0061]

[0062]

[0063]

[0064]

[0065] 2. Test products

[0066] In a specific embodiment, the detection kit includes: (1) reaction reagents for library construction, including: specific binding primers, universal primers, multiplex PCR polymerase, DNA ligase, end repair enzymes, dNTPs, reaction Buffer; (2) Reagents for purifying PCR reaction product...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a primer composition, a product and a method for detecting a PKD1 variant single sperm. The primer composition comprises primers capable of specifically amplifying a PKD1 gene and SNP in a 2Mb upstream and downstream range of the PKD1 gene. The primer composition can be used for genetic marking of variant sperms and therefore distinguish high-pathogenicity-risk haplotypes of men.

Description

【Technical field】 [0001] The invention relates to the technical field of embryo transfer detection, in particular to a primer composition, product and method for detecting PKD1 variant single sperm. 【Background technique】 [0002] Monogenic or Mendelian disorders are primarily due to a single genetic variation. There are approximately 10,000 known monogenic disorders, and although individual monogenic disorders are rare, the combined global prevalence of all monogenic disorders at birth is approximately 1%, and they account for almost 20% of childhood mortality. Therefore, couples at increased risk of having a child with a monogenic disorder can mitigate this risk through reproductive selection. Prenatal diagnosis can determine the genetic status of a developing pregnancy, however, patients who learn that their pregnancy is affected often face the difficult decision to terminate the pregnancy. Therefore, these patients may opt for preimplantation genetic testing (PGT). PG...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 曲宁费嘉张丽娜乔国枝
Owner PEKING JABREHOO MED TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap