Real-time fluorescent quantitative PCR detection kit and method for judging zinc deficiency state of areca-nut seedlings

Abstract

The invention belongs to the technical field of biology, and discloses a real-time fluorescent quantitative PCR detection kit and a method for judging the zinc deficiency state of areca-nut seedlings. The method comprises the steps that areca-nut zinc deficiency is carried out on an experimental base; samples are collected; ZIP family analysis and primer design are carried out; specificity verification is carried out on primers; and real-time fluorescent quantitative PCR detection is carried out. According to the real-time fluorescent quantitative areca-nut zinc deficiency diagnostic kit based on the ZIP family, the reasons of the iron deficiency and zinc deficiency state of the areca-nut seedlings can be determined only by providing the samples and detecting according to the provided method, and a basis is provided for subsequent prevention; ZIP family genes are obtained through differences of structural domains, and AcZIP genes capable of judging the zinc deficiency state of areca nuts are obtained or not through real-time fluorescent quantitative PCR verification; and the specificity of the primers is explained through primer gel imaging and a melting curve, and the reliability of results is explained through mutual verification of real-time fluorescent quantitative PCR results.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a real-time fluorescent quantitative PCR detection kit and a method for judging the zinc deficiency state of betel nut seedlings, in particular to a method for judging the zinc deficiency state of betel nut seedlings through the differential expression of AcZIP family genes. Background technique [0002] At present, zinc (Zn) is an essential trace element for plant growth and plays an irreplaceable role in plant growth and metabolism. Zinc ions mainly exist as protein complexes and activators of various proteins, and participate in a variety of life activities, including carbohydrate metabolism, protein synthesis, maintenance of cell membrane integrity, regulation of auxin synthesis, and pollen formation. Plants also need to regulate and maintain the expression of genes required for tolerance to environmental stresses, such as high light intensity and high temperature. M...

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Problems solved by technology

[0005] Through the above analysis, the existing problems and defects of the prior art are: in the prior art, there is no detailed detection of the state of zinc deficiency in betel nuts at the molecular level. The method of judging the zinc deficiency status of betel nut seedlings by differential expression of AcZIP gene family has not been reported yet

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