Antibody-dna conjugates and HPV detection and treatment
A conjugate and DNA sequence technology, applied in the field of antibody DNA conjugates and HPV detection and treatment, can solve the problems of low cost and scalability
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[0300] Embodiments (eg, of one or more methods, one or more systems, such as compositions, etc.) may additionally or alternatively include:
[0301] In some embodiments, the present disclosure provides a method of treating or preventing HPV 11 infection, comprising administering to a patient in need thereof a polypeptide comprising: (a) a sequence (and / or a sequence of sequences) comprising at least one of the following Suitable portions, such as epitopes of at least 7, optionally at least 8 contiguous amino acids): PVFITGSDF, LSTPQRLVT, FVVAVLGLL, TPFSPVTPA, LPVVIAFAV, LVAAENDTF, PSDSTVYVP, GAPEVVPPT, SDSTVYVPP, QGNTVEVKF, LVLTLLLYL, RVGLYSRAL, LILPVVIAF, TSSESTTPA, DSNVRLVVE、VLIILISDF、KPRARRRKR、VQIAAATTT、RRLFETREL、LTDAKVALL、MADDSALYE、EVVPVQIAA、NAVYELSDA、SSESTTPAI、QIAAATTTT、KIQSGVRAL、TVARTLGTL、STSATSIDQ、TSSLTITTS、ETNEDILKV、TVQSTTSSL、RALQQVQVT、LKDIVLDLQ、PVQIAAATT、YSIKKVNKT、PRARRRKRA、ASTSATSID、RKHRALTLI、ADDSALYEK、 KCKDIRSTL, STTSSLTIT, RKTACRRRL, VVIAFAVCI, AIAKRLDAC and / or MEV...
Embodiment 1
[0421] Example 1: Identification of epitopes from the proteome of HPV
[0422] As an example of the methods described herein, epitopes were identified from the proteomes of HPV types 6 (777 sequences), 11 (719 sequences), 16 (1278 sequences) and 18 (740 sequences) .
[0423] From those sequences, 3514 epitopes from the T cell prediction server were predicted. After discarding repetitive sequences, only 823 "de novo" epitopes were generated, which were aligned against proteins belonging to 148 species belonging to Lactobacillus and Fusobacterium nucleatum. Thus, 155 "common epitopes" between HPV and Lactobacillus proteins and 1 common epitope between HPV and F. nucleatum proteins were obtained. Those epitopes that did not meet the criteria described herein were discarded.
[0424] Of the 156 "de novo" epitopes, 5 of them also belong to bacteria known to be associated with HPV infection. They are: RAAKRRLFE epitope from HPV 16 and L. campylobacter, GGQTVQVYF and KGGQTVQVY ep...
Embodiment 2
[0496] Example 2: Identification of epitopes (777 segments) from the proteome of HPV6
[0497] From those sequences, a preliminary set of T-cell epitopes was obtained, which was subjected to the following multiple filtration processes: discarding repetitive sequences, matching 148 (and / or an appropriate number) of F. nucleatum and Lactobacillus The protein alignment of those species. Through this process, a set of common epitopes between HPV and Lactobacillus proteins was obtained. For example, an epitope (KNALTTAEI) shared between HPV type 6 and proteins from Fusobacterium nucleatum, Lactobacillus congeners, Lactobacillus fucoides, and Lactobacillus stevii was determined. Those epitopes that did not meet the criteria were discarded.
[0498] The list of epitopes may additionally or alternatively include those shown in the following table (Table 7). Additionally, the binding energies from the docking simulations, expressed in kcal / mol, are also listed. Binding energy can b...
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