IBS-D experimental animal model method for improving modeling rate and reducing death rate

Abstract

The invention provides an IBS-D experimental animal model method for improving the modeling rate and reducing the death rate, which selects an adult mouse as a modeling experimental subject, and comprises the following steps: step 1, colorectal dilation: firstly, slightly touching the anus part of the mouse to enable the mouse to defecate completely; plugging a trachea cannula with an air bag into the anus of the mouse, wherein the air bag end of the trachea cannula is located in the anus of the mouse, and the outer end of the trachea cannula is fixed to the root of the tail of the mouse; then placing the mouse in a transparent observation chamber which can limit the mouse to move left and right; and after the mouse is calm, injecting gas into the air bag of the trachea cannula to enable the air bag to have certain expansion pressure and stimulate the colorectum of the mouse; and step 2, chronic constraint stress: taking the mouse out of the transparent observation chamber, and fixing the abdomen and the four limbs of the mouse in the supine position. According to the method, the disease characteristics can be better and completely replicated, the death rate can be reduced, and the modeling rate is increased; and meanwhile, the molding effect can be more durable and stable.

Description

technical field [0001] The invention relates to the technical field of medical research, and relates to a method for establishing an animal model, in particular to an IBS-D (irritable bowel syndrome with diarrhea) experimental animal model method for improving the modeling rate and reducing the mortality rate . Background technique [0002] IBS (irritable bowel syndrome, irritable bowel syndrome) is a common functional bowel disease, mainly manifested as recurrent abdominal pain, accompanied by symptom relief after defecation, changes in defecation frequency, and abnormal stool properties. The incidence of IBS is relatively high, with a global prevalence of about 5% to 15%, the lowest in Southeast Asia (7.0%), and the highest in South America (21.0%). Women are more prevalent than men, and are more susceptible to chronic high-stress environments or childhood trauma. IBS is divided into diarrhea-predominant IBS (IBS-D), constipation-predominant IBS (IBS-C) and mixed IBS acc...

AI Technical Summary

Problems solved by technology

[0004] The pathogenesis of IBS is complex and diverse. Gastrointestinal motility and paresthesia are its pathophysiological basis. Its pathogenesis is related to the change of brain-gut axis function. It is a biological-psychological-social disorder. Therefore, it is difficult to establish an animal model.

The current modeling methods can construct the visceral sensitivity and abnormal motility characteristics of IBS-D to a certain extent, but they lack the manifestations of emotional disorders such as anxiety and depression, and cannot completely replicate the characteristics of the disease

At the same time, there is also a certain failure rate and mortality rate in the process of modeling

[0005] In summary, most of the existing animal models of irritable bowel syndrome start from a certain aspect of the pathophysiological mechanism, which has limitations to varying degrees, and there is a lack of accurate comparative studies on the advantages and disadvantages of the commonly used model preparation methods

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