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Detection primer composition for NLRP3 promoter methylation, application and detection method

A technology for detecting primers and promoters, which can be used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Achieve the effect of not easy to degrade, easy to store and transport, easy to operate

Pending Publication Date: 2021-09-21
HUNAN LINGKANG MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Because of this, the specific mechanism of action and signaling axis of agonist-receptor proteins have not been fully understood

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  • Detection primer composition for NLRP3 promoter methylation, application and detection method
  • Detection primer composition for NLRP3 promoter methylation, application and detection method
  • Detection primer composition for NLRP3 promoter methylation, application and detection method

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Embodiment Construction

[0032] Next, the technical scheme in the embodiment of the present invention will be described in conjunction with the embodiment of the present invention, which is apparent from the embodiments of the present invention, and not all of the embodiments of the invention, not all of the embodiments of the invention. Based on the embodiments of the present invention, there are all other embodiments obtained without making creative labor without making creative labor premises.

[0033] Moreover, the term "first", "second" is used only for the purpose of describing, and cannot be understood as an indication or implicit relative importance or implicitting the number of techniques indicated. Thereby, the features of "first", "second" may be defined, and one or more of this feature may be further included. In the description of the invention, the meaning of "multiple" is two or more, unless otherwise specifically defined.

[0034] In the description of the present specification, a descript...

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Abstract

The invention discloses a detection primer composition for NLRP3 promoter methylation, application and a detection method, the detection primer composition comprising an upstream primer cg21919599-F, a downstream primer cg21919599-Rbio and a sequencing primer cg21919599-S is designed, and the methylation level of the NLRP3 promoter cg21919599 is detected by utilizing a pyrosequencing technology. The detection method for the methylation level of the NLRP3 promoter is high in sensitivity and accuracy, and has important significance on pathological staging, early detection, prognosis and risk assessment of kidney cancer.

Description

Technical field [0001] The present invention belongs to the field of molecular biological detection, and more particularly to detection primer compositions, applications and detection methods of NLRP3 promoter methylation. Background technique [0002] Kidney cancer is one of the common malignant tumors that threaten people's health. The incidence of kidney cancer has been in a significant increase in the incidence of renovation in recent years, and its incidence rate is as high as one of my country's tumor incidence and the fastest growing tumor. Early kidney cancer is limited to the kidney envelope, and the 5-year survival period is as high as more than 90%; however, when the tumor diffuses into the envelope or distant transfer, the treatment of tumors is quite difficult and the prognosis is poor, and the 5-year survival is only available. Less than about 15%. More importantly, nearly 30% of patients have a first diagnosis due to early renal cancer often without clinical sympto...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869C12N15/11
CPCC12Q1/6886C12Q1/6869C12Q2600/154C12Q2600/112C12Q2600/118C12Q2531/113C12Q2565/301
Inventor 邓务国龙谦李振江
Owner HUNAN LINGKANG MEDICAL TECH CO LTD
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