Fungus flora related to esophageal squamous cell carcinoma morbidity, and screening method and application of fungus flora

A technology of esophageal squamous cell carcinoma and screening method, which is applied in the field of disease treatment and can solve the problems of insufficient representation and poor specificity

Inactive Publication Date: 2021-09-24
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This study is to isolate and cultivate known fungi, and only discusses specific fungi, which has poor specificity and insufficient representation

Method used

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  • Fungus flora related to esophageal squamous cell carcinoma morbidity, and screening method and application of fungus flora
  • Fungus flora related to esophageal squamous cell carcinoma morbidity, and screening method and application of fungus flora
  • Fungus flora related to esophageal squamous cell carcinoma morbidity, and screening method and application of fungus flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Obtaining tissue samples of esophageal squamous cell carcinoma

[0053] Samples from 66 patients with esophageal squamous cell carcinoma who were treated in Fujian Cancer Hospital and Zhangzhou Hospital in Fujian Province from February 2013 to October 2017 were selected, among which the cancer tissues were from 39 patients and the paracancerous tissues were from 53 patients.

[0054] All cancer tissue samples were diagnosed as esophageal squamous cell carcinoma by pathological hematoxylin-eosin staining (HE), and no tumor cells were detected in the paracancerous tissue samples.

[0055] 2 Genomic DNA extraction and detection

[0056] (1) Prepare lysozyme buffer. Prepare as shown in Table 1 below, invert and mix well, add to Millipore Amicon Ultra-4 Centrifugal Filter and centrifuge at 10,000 rpm for 20 minutes, then dispense into sterile, DNA-free EP tubes, and store in a -20°C refrigerator.

[0057] The formula of table 1 lysozyme buffer

[0058]

[0059] (2) ...

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Abstract

The invention provides a fungal flora related to esophageal squamous carcinoma attack and a screening method and application thereof, and belongs to the technical field of disease treatment. According to the present invention, 10 types of abundance-difference flora, including Prussiaporsica, Fusariumsolani, Nigrosporazole, Acremonium, Tausoniapullatus, Hanseniasporazole, Spegazazole, Rhodotorula, Golovinobacteria and Malassezidazole, are screened from esophageal squamous cell carcinoma cancer tissues and para-carcinoma tissues, and can be used for the comprehensive diagnosis or the auxiliary diagnosis of an esophageal squamous cell carcinoma morbidity situation.

Description

technical field [0001] The invention belongs to the technical field of disease treatment, and in particular relates to a fungal flora related to the pathogenesis of esophageal squamous cell carcinoma and its screening method and application. Background technique [0002] The connecting organ from the mouth to the stomach is the esophagus, and the primary tumor of the esophagus is called esophageal cancer. Esophageal squamous cell carcinoma is a kind of esophageal cancer, which is a malignant tumor derived from esophageal squamous epithelial mucosal cells. Esophageal cancer is a high-incidence type in China, and the incidence of esophageal adenocarcinoma is very low. Other esophageal cancers include esophageal melanoma, small cell carcinoma, and undifferentiated carcinoma, but the incidence of squamous cell carcinoma is relatively high, accounting for more than 90% to 95%. [0003] Currently, esophageal squamous cell carcinoma is treated with surgery-based comprehensive trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/6895C12Q1/06C12Q1/04C12R1/77C12R1/645
CPCC12N1/14C12Q1/6895C12Q1/06C12Q1/04G01N2800/7028
Inventor 胡志坚林征饶雯清刘双
Owner FUJIAN MEDICAL UNIV
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