Virus neutralizing antibody and non-neutralizing antibody combined detection method, detection card and application

An antibody detection and combined detection technology, applied in the field of biomedical detection, can solve the problems of low sensitivity, poor specificity, and inability to distinguish neutralizing antibodies and non-neutralizing antibodies in the detection of neutralizing antibodies, achieving fast detection speed and improving specificity. , the effect of high sensitivity

Active Publication Date: 2021-09-24
NANJING LEADING BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of low detection sensitivity, poor specificity and inability to distinguish neutralizing antibodies from non-neutralizing antibodies in the prior art, and provide a simple, fast, highly sensitive and specific virus neutralization Combined detection method of antibody and non-neutralizing antibody, detection card and application thereof

Method used

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  • Virus neutralizing antibody and non-neutralizing antibody combined detection method, detection card and application
  • Virus neutralizing antibody and non-neutralizing antibody combined detection method, detection card and application
  • Virus neutralizing antibody and non-neutralizing antibody combined detection method, detection card and application

Examples

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Embodiment 1

[0081] A detection card for SARS-CoV-2 neutralizing antibody and non-neutralizing antibody, the detection card includes a chromatographic test paper, a housing and a sample diluent, and the chromatographic test paper includes a bottom plate, a sample pad 1, a binding pad 2, NC membrane 3 and water-absorbing pad 4, the sample pad 1, binding pad 2, NC membrane 3 and water-absorbing pad 4 are sequentially pasted on one side of the bottom plate; the NC membrane 3 is sequentially provided with non-neutralizing antibody detection lines 5, Neutralizing antibody detection line 6 and quality control line 7, the non-neutralizing antibody detection line 5 is close to the binding pad 2, the quality control line 7 is close to the water-absorbing pad 4, and the non-neutralizing antibody detection line 5 is coated with RBD - ACE2 protein binding complex, the detection line 6 is coated with RBD protein; the binding pad is provided with RBD protein markers and chicken IgY markers, the label car...

Embodiment 2

[0091] A detection card for SARS-CoV-2 neutralizing antibody and non-neutralizing antibody, comprising chromatography test paper, housing and sample diluent, said chromatography test paper comprising bottom plate, sample pad, binding pad, NC membrane and water-absorbing pad , the sample pad, binding pad, NC membrane and water-absorbent pad are pasted on one side of the bottom plate in sequence; the NC membrane is provided with a non-neutralizing antibody detection line, a neutralizing antibody detection line and a quality control line in sequence, and the non-neutralizing antibody detection line and quality control line are sequentially arranged on the non-neutralizing antibody The neutralizing antibody detection line is close to the binding pad, the quality control line is close to the water-absorbing pad, the non-neutralizing antibody detection line is coated with RBD-ACE2 protein binding complex, and the neutralizing antibody detection line is coated with RBD protein; The bi...

Embodiment 3

[0095] A detection card for SARS-CoV-2 neutralizing antibody and non-neutralizing antibody, comprising chromatography test paper, housing and sample diluent, said chromatography test paper comprising bottom plate, sample pad, binding pad, NC membrane and water-absorbing pad , the sample pad, binding pad, NC membrane and water-absorbent pad are pasted on one side of the bottom plate in sequence; the NC membrane is provided with a non-neutralizing antibody detection line, a neutralizing antibody detection line and a quality control line in sequence, and the non-neutralizing antibody detection line and quality control line are sequentially arranged on the non-neutralizing antibody The neutralizing antibody detection line is close to the binding pad, the quality control line is close to the water-absorbing pad, the non-neutralizing antibody detection line is coated with RBD-ACE2 protein binding complex, and the neutralizing antibody detection line is coated with RBD protein; The bi...

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Abstract

The invention relates to a virus neutralizing antibody and non-neutralizing antibody combined detection method, a detection card and an application of the method. A neutralizing antibody is detected according to a virus receptor binding protein sandwich method principle, and the neutralizing antibody is a compound formed by a protein combined with a virus receptor and a protein which can block the binding with the neutralizing antibody. A non-neutralizing antibody of the targeted receptor protein is captured in advance, so that the specificity of the neutralizing antibody detected by a sandwich method subsequently is ensured. The problems that in the prior art, the neutralizing antibody detection sensitivity is low, the specificity is poor, and the neutralizing antibody and the non-neutralizing antibody cannot be distinguished are solved, and the virus neutralizing antibody and non-neutralizing antibody combined detection method which is simple, convenient, rapid, high in sensitivity and high in specificity, the detection card and the application of the detection method are provided.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a highly sensitive and highly specific combined detection method for virus neutralizing antibodies and non-neutralizing antibodies, a detection card and applications thereof. Background technique [0002] When the virus infects humans, the receptor-binding protein on the surface of the virus will bind to the receptor on the surface of the host cell, thereby entering the host cell, causing symptoms such as fever and infection in the host. After the human body is invaded by a virus or vaccinated, it will produce antibodies through the immune response, and some of the antibodies can specifically bind to the receptor binding region on the surface of the virus, thereby preventing the virus from entering the host cell, which is a neutralizing antibody; while other antibodies produced It can bind to the virus, but cannot block the receptor binding protein on the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N33/558G01N33/58G01N33/531G01N33/53
CPCG01N33/6854G01N33/56983G01N33/558G01N33/587G01N33/585G01N33/582G01N33/531G01N33/5302G01N2469/20G01N2333/165G01N2333/08G01N2333/115
Inventor 张玉基王鹏王倩王维徐达叶赟
Owner NANJING LEADING BIOMEDICAL TECH CO LTD
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