Deterministic lateral displacement micro-fluidic chip for separating magnetic beads from in-vitro blood

Abstract

The invention discloses a deterministic lateral displacement micro-fluidic chip for separating magnetic beads from in-vitro blood, and the deterministic lateral displacement micro-fluidic chip comprises inlets, outlets, and a separation cavity and a filtering cavity which are connected between the inlets and the outlets, wherein the inlets comprise a mixed liquid inlet for conveying mixed liquid to the separation cavity and two groups of buffer liquid inlets for conveying buffer liquid into the separation cavity, and the two groups of buffer liquid inlets are respectively positioned on two sides of the mixed liquid inlet; the outlets comprise a waste liquid outlet for discharging waste liquid and a blood outlet for discharging clean blood; the separation cavity is a separation area formed by a plurality of groups of triangular micro-columns which are arranged in a staggered manner; the filtering cavity is a filtering area formed by a plurality of groups of capturing units. According to the deterministic lateral displacement micro-fluidic chip for separating magnetic beads from in-vitro blood, the magnetic beads can be separated from the blood in a high-flux flow velocity state, and meanwhile, the magnetic beads and magnetic bead fragments are prevented from returning to the blood.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a deterministic lateral displacement microfluidic chip for separating magnetic beads from extracorporeal blood. Background technique [0002] Cancer has become one of the leading causes of death of the population in the country. The vast majority of cancer patients do not die from the original tumor, but the main cause of death is when the original tumor has metastasized to other body parts and causes organ failure. One of the main mediators is circulating tumor cells in the blood, that is, tumor cells that break off from the original tumor and invade blood vessels. When circulating tumor cells leave the blood vessels and invade other tissues, they will form a new metastasis, which will grow to a Metastatic tumors, therefore, the capture of circulating tumor cells is an important area of ​​clinical oncology at present; [0003] At present, the mainstream method of capturing cir...

AI Technical Summary

Problems solved by technology

[0005] Existing microfluidic technologies for the separation of circulating tumors all focus on the direct separation of circulating tumor cells, and have not yet separated the tumor cell-magnetic bead complex adsorbed on the magnetic beads.

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