KASP molecular marker closely linked with major QTL of pod wall tenderness of peas and development method and application of KASP molecular marker

A molecular marker, pea pod technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc. Select the effect of precision

Active Publication Date: 2021-10-08
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]However, limited by the complexity of pea omics research and pod wall formation, molecular markers related to

Method used

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  • KASP molecular marker closely linked with major QTL of pod wall tenderness of peas and development method and application of KASP molecular marker
  • KASP molecular marker closely linked with major QTL of pod wall tenderness of peas and development method and application of KASP molecular marker
  • KASP molecular marker closely linked with major QTL of pod wall tenderness of peas and development method and application of KASP molecular marker

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Example Embodiment

[0044] In order to understand the technical content of the present invention, the following examples are detailed in detail below.

[0045] The terms used in the present invention have generally known to those of ordinary skill in the art, unless otherwise stated. In an embodiment, various processes and methods described in detail are common methods well known in the art.

[0046] The pea material used in the following examples is preserved by China Metrology and can be used in the art and researchers. Other consumables, reagents, etc., if there is no special instructions, are obtained from the business pathway.

[0047] 1) Construction and genetic analysis of group materials

[0048] During the preliminary study, the inventors were preserved by the hard pod pea variety ("PS71", which was stored and provided by the China Metrology University Plant Quality Safety Biology Laboratory ("PS35", from China Observing and offering of a plant quality safety biology laboratory found that bo...

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Abstract

The invention provides a KASP molecular marker closely linked with a major QTL of pod wall tenderness of peas. The KASP molecular marker comprises a first KASP molecular marker and/or a second KASP molecular marker, wherein the first KASP molecular marker is used for specifically detecting a 297000666 site of a Chr1LG6 chromosome of the peas, and the second KASP molecular marker is used for specifically detecting a 302006525 site of the Chr1LG6 chromosome of the peas. The invention further provides a related development method and application. The KASP molecular marker closely linked with the major QTL of the pod wall tenderness of the peas, provided by the invention, is closely linked with the major QTL of the pod wall tenderness of the peas, so that the pod wall tenderness can be quickly and accurately pre-judged at an early growth stage of the peas, molecular marker-assisted selection with a soft pod type or a hard pod type as a breeding target is realized, the accuracy of breeding selection is improved, the breeding process is accelerated, and thus, the KASP molecular marker has an important application value in quality improvement on edible pea pods and is applicable to large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of bioinformatics and molecular marker genetics, more specifically, to the field of pea KASP molecular marker technology, in particular to a KASP molecular marker closely linked with the main effect QTL of pea pod wall tenderness and its development method and apply. Background technique [0002] Pea (Pisum sativum L.) belongs to the genus Pea of ​​the family Fabaceae. It is one of the four most important soybean crops in the world and the second largest edible bean in my country. Its planting area covers almost all parts of the country. According to the type of pod wall and edible parts, peas are divided into grain peas and pod-eating peas. According to the statistics of the Food and Agriculture Organization of the United Nations, the total annual output of peas in my country reaches 14.5 million tons, accounting for more than 40% of the world's total output (FAOSTAT 2020). [0003] The pod is the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11G16B20/20
CPCC12Q1/6895G16B20/20C12Q2600/13C12Q2600/156
Inventor 王永刚徐沛
Owner CHINA JILIANG UNIV
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