Blood coagulation analyzer reagent calibration method based on immunoturbidimetry

Abstract

The invention discloses a blood coagulation analyzer reagent calibration method based on immunoturbidimetry, and the method comprises the following steps: carrying out multiple experiments by using calibration samples with different concentrations of measured components according to the direct proportion of absorbance difference to the concentration of the calibration samples, calculating the absorbance difference of plasma samples with different concentrations of measured components, respectively adopting a two-point method and a rate method to calculate absorbance difference values for calibration samples with different concentrations of measured components, and respectively obtaining two calibration curves. According to the reagent calibration method for the blood coagulation analyzer based on the immunoturbidimetry, two different calibration curves are used on the basis of combining actual clinical application conditions and experimental experience accumulation aiming at detected component samples with different concentrations, and two calibration methods are adopted, so that the calibration method is more flexible, accurate and high in applicability.

Description

technical field [0001] The invention relates to the technical field of coagulation detection, in particular to a reagent calibration method of a coagulation analyzer based on immunoturbidimetry. Background technique [0002] Immunoturbidimetry is a commonly used coagulation detection method, which is specially used to detect the reaction of liquid turbidity change caused by latex agglutination. [0003] The test principle is: the sample and mouse anti-human monoclonal antibody latex particles react in the test cup to form antigen-antibody complex particles, resulting in turbidity changes. The light source and receiver are arranged at 90 degrees on both sides of the test cup, which can detect the absorbance change of the liquid in the test cup. The absorbance has a correlation with the liquid turbidity, and the change of the optical signal is received and converted into an electrical signal, which is then amplified and calculated. [0004] For the coagulation analyzers and ...

AI Technical Summary

Problems solved by technology

The same method and experimental conditions may not be applicable to different brands of coagulation analyzers or coagulation reagents

Moreover, the coagulation analyzers and coagulation reagents currently on the market only use a single method (including a single parameter or calculation method) in calibration, which cannot accurately express the characteristics of the response curves of the components or concentrations of different samples to be tested.

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