Molecular marker C27449 for rapidly identifying genetic sex of penaeus japonicus and application of molecular marker C27449

The technology of C27449R and C27449F is applied in the field of molecular marker C27449 for rapid identification of the genetic sex of penaeus japonicus, which can solve the problem of lack of sex control technology of penaeus japonicus, and achieve the effects of simple and quick sex identification, low sample requirements and broad application prospects.

Active Publication Date: 2021-10-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no efficient tec

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker C27449 for rapidly identifying genetic sex of penaeus japonicus and application of molecular marker C27449
  • Molecular marker C27449 for rapidly identifying genetic sex of penaeus japonicus and application of molecular marker C27449

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. DNA extraction

[0025] The DNA of Penaeus japonicus was extracted by Novogene, 30 male and 30 individuals.

[0026] 2. DNA pool construction

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a molecular marker C27449 for rapidly identifying genetic sex of penaeus japonicus and application of the molecular marker C27449. The nucleotide sequence of the molecular marker C27449 is shown as SEQ ID No.1, and the nucleotide sequences of a primer pair used for detecting the molecular marker C27449 are respectively as follows: C27449F: TCATCCAAGTTCCAGCCT and C27449R: GGTCCCCCACAACATACA. The molecular marker C27449 can not be influenced by tissue specificity and environment of penaeus japonicus, use steps are simple, results are obvious, genomic DNA of the penaeus japonicus and a primer pair are subjected to PCR (polymerase chain reaction) and then electrophoresis detection, if a strip appears at 120bp, the penaeus japonicus is female penaeus japonicus, if no strip exists, the penaeus japonicus is male penaeus japonicus. The molecular marker can also promote the establishment of a penaeus japonicus all-female or high-female fry breeding technology, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers, in particular to a molecular marker C27449 for quickly identifying the genetic sex of Penaeus japonicus and its application. Background technique [0002] Japanese prawns ( Penaeus japonicus ) belongs to Crustacea, Decapod, Prawnaceae, and Penaeus, also known as car shrimp, flower shrimp, flower tail shrimp, etc., and is mainly distributed in the coastal areas south of Jiangsu in my country. Penaeus japonicus has the characteristics of rapid growth, strong disease resistance, long survival time in dry dew, and high survival rate in long-distance transportation. Because of its high economic value and high market price, it is an important economic product in coastal areas of my country. types of prawns. During the breeding process, the mature female shrimp of Penaeus japonicus is larger than the male shrimp. Therefore, if efficient parthenoculture can be realized, the economic benefi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/6879C12Q1/686C12Q2565/125Y02A40/81
Inventor 兰伟康吕建建金玲刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap