A method for predicting the tertiary structure of circular nucleic acid aptamers targeting biotoxins

Abstract

The invention provides a method for predicting the tertiary structure of a biotoxin-targeting circular nucleic acid aptamer. After obtaining the nucleotide sequence of a biotoxin-targeting nucleic acid aptamer, constructing a biotoxin-targeting circular The tertiary structure model of circular nucleic acid aptamers can be used to predict the tertiary structure of circular nucleic acid aptamers targeting biological toxins. After adopting the above technical scheme, the tertiary structure of the circular nucleic acid aptamer can be predicted from its sequence. The tertiary structure of the circular nucleic acid aptamer simulated in this application has excellent accuracy and stability, and can adjust the accuracy of prediction to predict the circular topology according to the needs of users.

Description

technical field [0001] The invention belongs to the technical field of nucleotide tertiary structure prediction, and in particular relates to a method for predicting the tertiary structure of a circular nucleic acid aptamer targeting biological toxins. Background technique [0002] Nucleic acid aptamers are functional nucleotides screened from random libraries through systematic evolution of ligand index enrichment (Systematic Evolution of Ligands by Exponential Enrichment, SELEX), with high specificity and a wide range of optional target molecules , has shown broad application prospects in basic research, clinical diagnosis and treatment. However, most aptamers are not stable and are easily degraded by ribozymes in vivo, which makes it difficult for nucleic acid aptamers to be used in therapy. With the deepening of aptamer research, we found that circular DNA The aptamer has a more stable structure, can resist the degradation of ribozymes, and can also stabilize high-affin...

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Problems solved by technology

However, due to high cost and long time-consuming, they cannot be well applied to cyclized aptamers. With the rapid development of computer technology, people have been able to predict the tertiary structure of aptamers by computer, but existing The accuracy of the predicted model is often not high. This is because many prediction software are difficult to analyze some more complex systems, and the aptamer molecules often have greater flexibility, which makes the differences of aptamers in different environments It is quite large, and it also brings great difficulties to the prediction of circular aptamers, and most of the software cannot predict the circular structure of DNA, so there is no one that can be used for circular aptamers Techniques for Tertiary Structure Prediction

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