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Producing isothiocyanates from callus suspension cultures

A technology of isothiocyanate and suspension culture, applied in tissue culture, plant regeneration, cell culture medium, etc., can solve the problems of large reagent dosage, cumbersome overall operation, and not suitable for industrial production

Inactive Publication Date: 2021-10-29
比贝婷作物科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall operation is cumbersome and the dosage of reagents is large, which makes this method more suitable for experimental reactions, but not suitable for industrial production

Method used

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  • Producing isothiocyanates from callus suspension cultures
  • Producing isothiocyanates from callus suspension cultures
  • Producing isothiocyanates from callus suspension cultures

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Embodiment Construction

[0052] While exemplary embodiments are described below, the present invention is only limited by the appended claims. The appended claims are hereby expressly incorporated into this Detailed Description of the Invention, with each claim and each combination permitted by the structural dependencies defined by the claims forming a separate embodiment of the invention.

[0053] The word "comprising" used in the claims is not limited to the features, elements or steps stated thereafter, and does not exclude other features, elements or steps. Accordingly, this prescribes the presence of the mentioned features, but does not exclude the further presence or addition of one or more features.

[0054] In this detailed description, various specific details are set forth. Embodiments of the invention may be practiced without these specific details. Furthermore, well-known features, elements and / or steps have not necessarily been described in detail for clarity and conciseness of the pre...

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Abstract

The present invention provides a method of producing isothiocyanates, The method comprises forming, in a semi-solid or solid callus induction medium, compact callus aggregates from cells obtained from explant material of a Brassica oleracea L. plant. The method comprises transferring cells from the callus aggregates, e.g. transferring the callus aggregates, to a suspension culture in a liquid medium in a shake flask, the liquid medium containing a plurality of elicitors. The method comprises transferring, after culturing in the shake flask, cells from the suspension culture to a further suspension culture in a bioreactor containing the elicitors. The method comprises extracting and / or purifying of at least one isothiocyanate from cells obtained from the bioreactor. The elicitors comprise chitosan and salicylic acid to increase accumulation of benzyl isothiocyanate or any other isothiocyanate.

Description

technical field [0001] The present invention relates to the field of in vitro production of isothiocyanates, ie using plant cell cultures. More specifically, the present invention relates to methods for the production of isothiocyanates from cellular material of the order Brassicales and their use. Background technique [0002] Isothiocyanate, whose general formula is r-N=C=s structure, is an important functional substance in natural products and pharmaceutically active compounds. These compounds are important intermediates in organic synthesis and are widely used in industries such as medicine, nutrition, cosmetics and agriculture. Isothiocyanate can participate in a variety of organic reactions for the synthesis of various types of compounds containing sulfur, nitrogen, and oxygen, especially heterocyclic compounds. Isothiocyanate can be widely used in the preparation of organic synthetic products, such as pharmaceuticals, cosmetic raw materials, pesticides, dyes, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H6/20A01H4/00C12N5/04
CPCA01H4/00A01H6/203C12N5/04C12N5/0025C12N2501/90C12P13/00
Inventor J·霍芬克J·托池
Owner 比贝婷作物科学有限公司