Oocyte in-vitro maturation culture solution and application thereof

A technology for in vitro maturation and culture of oocytes, applied in the direction of cell culture active agents, germ cells, tissue culture, etc., can solve the problem of lack of clearance, restrictions on the efficient production and application of parthenogenetic embryos of somatic cell nuclear transfer embryos, poor quality of oocytes, etc. problem, achieve the effect of reducing oxidative stress response, improving oocyte quality, improving efficiency and quality

Pending Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0003] Due to the influence of internal environment and many factors in the process of oocyte maturation in vivo, the core factors affecting oocyte maturation are not yet known
Therefore, in the in vitro maturation culture of oocytes, due to the lack of some unknown essential components, the quality of in vitro matured oocytes is much lower than that of normal matured oocytes in vivo, which l

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  • Oocyte in-vitro maturation culture solution and application thereof
  • Oocyte in-vitro maturation culture solution and application thereof

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Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0020] 1. Quantitative proteomic detection of follicular fluid

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Abstract

The invention discloses a porcine oocyte in-vitro maturation culture solution added with a chemotactic factor ligand 14 (CXCL14). The culture solution can improve the quality of in-vitro maturation of oocytes. The quality of the oocytes is closely related to a parthenogenetic activation technology, an in-vitro fertilization technology and a somatic cell cloning technology, and improvement of the quality of the in-vitro mature oocytes plays an extremely important role in the fields of livestock production, medical reproduction, life science research and the like.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a culture medium for in vitro maturation of oocytes and an application thereof. Background technique [0002] In vitro matured oocytes can be used to prepare cloned embryos, in vitro fertilized embryos, parthenogenetically activated embryos, etc. The preparation of these embryos is closely related to many scientific researches, and has important uses in the fields of animal husbandry production, medical reproduction, and life science research. . However, in actual preparation, due to problems such as acquisition cost and technology, in vitro matured oocytes cannot be produced in large quantities and are of poor quality, so technologies such as somatic cell nuclear transfer cannot be widely used in various fields of scientific research and production. Somatic cell nuclear transfer technology is to transfer donor cells into enucleated in vitro mature oocytes, so that the gene...

Claims

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/21
Inventor 吴珍芳张宇星李紫聪蔡更元李亚楠张贤君招华兴梁雅琳郑恩琴徐铮顾婷黄思秀杨杰杨化强洪林君
Owner SOUTH CHINA AGRI UNIV
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