Application of Maize Casein Kinase 2ck2α2 and its Encoding Gene Based on High Temperature Stress Response

A protein kinase and gene encoding technology, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve the problems of high temperature resistance genetic resources and lack of germplasm resources in maize.

Active Publication Date: 2022-05-31
HENAN AGRICULTURAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a corn casein kinase 2CK2α2 and the application of its coding gene based on high temperature stress response, so as to solve the current technical problem of lack of high temperature resistant gene resources and germplasm resources of corn

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Maize Casein Kinase 2ck2α2 and its Encoding Gene Based on High Temperature Stress Response
  • Application of Maize Casein Kinase 2ck2α2 and its Encoding Gene Based on High Temperature Stress Response
  • Application of Maize Casein Kinase 2ck2α2 and its Encoding Gene Based on High Temperature Stress Response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Casein kinase 2 in response to high temperature in corn α Identification of subunits

[0024] 1. Identification of casein kinase 2 affected by high temperature in maize by qRT-PCR α subunit

[0025] To determine ZmCK2α1 、 α2 、 α3 、 Whether α4 is affected by high temperature, the three-leaf stage seedlings of the maize hybrid Zhengdan 958 were treated with high temperature stress for 0 h, 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h and 10 h respectively, and then the third The total RNA of the two leaves was analyzed by qRT-PCR. The results showed that ZmCK2α2 and ZmCK2α3 were significantly induced by high temperature stress, and the expression of ZmCK2α2 increased most significantly. and ZmCK2α4 expression had little effect (see figure 1 ).

[0026] 2. ZmCK 2 α 2 Analysis of cis-acting elements in the promoter region of genes

[0027] For further analysis ZmCK2α2 Whether it responds to stress, the cis-acting elements of its promoter were analyzed. First fou...

Embodiment 2

[0029] Embodiment 2: ZmCK 2 α 2. High temperature resistance function test of genes

[0030] Malondialdehyde (MDA) content is the embodiment of the degree of peroxidation of plant cell membranes. The high content of malondialdehyde indicates that the degree of peroxidation of plant cell membranes is high and the cell membrane is seriously damaged. while H 2 O 2 The accumulation of content can directly or indirectly oxidize biological macromolecules such as nucleic acids and proteins in cells, and damage the cell membrane, thereby accelerating the aging and disintegration of cells. Catalase (CAT) can scavenge H 2 O 2 , is one of the important enzymatic defense systems in plants. for sure ZmCK2α2 Whether it is involved in the resistance of maize to high temperature, using the maize protoplast transient transformation system, the overexpression vector OE -ZmCK 2 α 2 and ZmCK 2 α The double-stranded RNA of 2 and distilled water were transformed into maize leaf protop...

Embodiment 3

[0032] Example 3: Based on ZmCK 2 α Characterization of high temperature resistance function of 2 transgenic plants

[0033] To further explore ZmCK 2 α 2 The function under high temperature stress was obtained by maize genetic transformation technology. ZmCK 2 α 2 Overexpression homozygous lines. First, by homologous recombination ZmCK 2 α 2 Recombination into the binary expression vector pFGC5941 containing the 35S promoter, then transform the plasmid with the correct sequencing result into Agrobacterium GV3101, and finally use the Agrobacterium-mediated in situ transformation of maize mature embryos to transform pFGC5941- ZmCK2α2 The vector is transformed into maize, and T is obtained after herbicide screening 0 Generation positive plants, and then selfed to T 2 generation and obtained the ear of the transgenic plant.

[0034] 1. Detection of transgenic lines by qRT-PCR ZmCK 2 α 2 expression

[0035] To identify overexpressed transgenes in ZmCK 2 α Wh...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a corn casein kinase 2CK2α2 and the application of its coding gene based on high temperature stress response, aiming at solving the technical problem of lack of high temperature resistant gene resources and germplasm resources of current corn. The present invention is based on maize casein kinase 2 α The discovery that the subunit can respond to high temperature stress constructed the overexpression vector OE ‑ZmCK 2 α 2 、 pFGC5941 ‑ZmCK 2 α 2 Overexpression vector, obtained by using maize genetic transformation technology wxya 2 α 2 Overexpress homozygous lines, and use qRT-PCR detection to screen high-expression lines as breeding materials for high-temperature-resistant maize varieties / lines. The present invention identifies maize casein kinase 2α The subunit can respond to high temperature stress, and its mechanism of action under high temperature stress has been preliminarily analyzed, which is helpful to understand the heat resistance mechanism of maize, and can provide important gene resources and related germplasm resources for breeding high temperature resistant maize varieties.

Description

technical field [0001] The invention relates to the technical field of high temperature stress resistance in maize, in particular to the application of maize casein kinase 2CK2α2 and its encoding gene based on high temperature stress response. Background technique [0002] Corn is the largest grain crop in my country, and its output and area are both the largest grain crops, and it plays an important role in national food security. However, with the warming of the global climate, high temperature and heat damage have occurred frequently in recent years, causing serious losses to corn production. Relevant studies have shown that the optimum temperature for maize reproductive growth is 25-28 °C. Higher temperatures can accelerate its growth and development and shorten its growth period, but extreme high temperatures hinder its growth and development. For example, high temperatures above 32 °C are not conducive to Its dry matter accumulation, and the high temperature above 35 ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/82A01H5/00A01H6/46C12Q1/6895
CPCC12N9/12C12Y207/11001C12N15/8271C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 胡秀丽刘艳培赵玉龙陈闪闪
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap