Biological microscopic vision pre-focusing device and method based on spectral confocal principle

Abstract

The invention discloses a biological microscopic vision pre-focusing device and method based on a spectral confocal principle. The device comprises a spectrum confocal sensing module, a microscopic optical module, a slide clamping device, a focusing motion device and a scanning motion device. The spectrum confocal sensor measures the distance to the upper / lower surface of the cover glass and the thickness of a biological tissue layer to be observed at one time; the spatial relation between the object space focus of the microscope objective and the measured value of the spectrum confocal sensor is determined through calibration, and then the measured value of the spectrum confocal sensor directly drives the focusing motion device to achieve accurate automatic focusing of biological microscopic vision. The invention further provides a digital slice scanner based on the device and the method. The method is suitable for the construction of the focused topographic map of the digital slice scanner, can also be used for the automatic focusing of the biological digital microscope, and has the characteristics of high focusing speed, accurate layered focus prediction and the like.

Description

technical field [0001] The invention belongs to the field of automatic focusing, and in particular relates to an automatic focusing device and method of a biological microscopic vision system based on the principle of spectral confocal. The invention can be used in equipment and instruments based on biological microscopic imaging, and is especially suitable for fast pre-focusing of digital slide scanners. Background technique [0002] The digital slice scanner uses a digital camera system to scan and collect traditional glass pathological slices to obtain high-resolution digital images showing the status of the patient's cells and tissues. The obtained images are automatically spliced ​​and processed with high precision and multiple fields of view to obtain high-quality Whole Slide Image (WSI). By sharing WSI data, remote pathological diagnosis and image reading exchange can be realized, which is conducive to the sharing and utilization of high-quality medical expert resour...

AI Technical Summary

Problems solved by technology

These technologies have two obvious disadvantages: one is improper selection of feature points, which is prone to blurred images in large areas, which may affect the diagnosis; the other is that the process of finding the focus needs to drive the focus movement device to move and collect images synchronously. Calculating the focus position takes a lot of time

The cause of the first shortcoming is complex. The details of the field of view under the microscope under the feature point are not sufficient or foreign objects in the non-tissue area can cause focusing errors, which is difficult to avoid. The second shortcoming makes pre-focusing almost account for one-third of the entire scanning time. one

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