A biological microscope vision pre-focusing device and method based on the principle of spectral confocal

Abstract

The invention discloses a biological microscope vision pre-focusing device and method based on the principle of spectral confocal. The device includes a spectral confocal sensing module, a microscopic optical module, a glass slide holding device, a focusing movement device and a scanning movement device. The spectral confocal sensor measures the distance to the upper / lower surface of the cover glass and the thickness of the biological tissue layer to be observed at one time; through calibration, the spatial relationship between the objective focus of the microscope objective and the measured value of the spectral confocal sensor is established, thereby realizing The focusing motion device is directly driven by the measurement value of the spectral confocal sensor to realize the precise automatic focusing of biological microscope vision. The present invention also provides a digital slice scanner based on the above device and method. The invention is suitable for the construction of the focal topographic map of the digital slice scanner, and can also be used for the automatic focusing of the biological digital microscope, and has the characteristics of fast focusing speed, accurate layered focus prediction and the like.

Description

technical field [0001] The invention belongs to the field of automatic focusing, and in particular relates to an automatic focusing device and method of a biological microscope vision system based on the principle of spectral confocal. The invention can be used for equipment and instruments based on biological microscopic imaging, and is especially suitable for fast pre-focusing of digital slice scanners. Background technique [0002] The digital slice scanner uses a digital camera system to scan and collect traditional glass pathological slices to obtain high-resolution digital images showing the condition of the patient's cell tissue. Whole Slide Image (WSI). By sharing WSI data, remote pathological diagnosis and image reading exchange can be realized, which is conducive to the sharing and utilization of high-quality medical expert resources. [0003] The imaging quality of digital slide scanners relies on high-precision focus control, so the autofocus technology of the ...

AI Technical Summary

Problems solved by technology

These technologies have two obvious disadvantages: one is improper selection of feature points, which is prone to blurred images in large areas, which may affect the diagnosis; the other is that the process of finding the focus needs to drive the focus movement device to move and collect images synchronously. Calculating the focus position takes a lot of time

The cause of the first shortcoming is complex. The details of the field of view under the microscope under the feature point are not sufficient or foreign objects in the non-tissue area can cause focusing errors, which is difficult to avoid. The second shortcoming makes pre-focusing almost account for one-third of the entire scanning time. one

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