Lactobacillus amylovorus and application thereof

A technology of Lactobacillus amylovora and bacteria agent, applied in the field of probiotics, can solve the problems of not exploring the potential of Lactobacillus amylovora antibiotic substitutes, achieve good in vitro probiotic properties, high lactic acid production capacity, and improve the effect of feed conversion rate

Active Publication Date: 2021-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In domestic research, there is no research on the potential of Lactobacillus amylovora as a substitute for antibiotics

Method used

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  • Lactobacillus amylovorus and application thereof
  • Lactobacillus amylovorus and application thereof
  • Lactobacillus amylovorus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Isolation and Identification of Lactobacillus amylovorus SLZX20-1

[0054] 1. Isolation of Lactobacillus amylovora

[0055] Lactobacillus amylovorus (Lactobacillus amylovorus) SLZX20-1 was isolated from the feces of Tibetan pigs from the Tibetan Pig Breeding Cooperative in Longzi County, Shannan City, Tibet Autonomous Region. The specific isolation method is as follows:

[0056] Take out about 1g of Tibetan pig feces samples stored in a -80°C refrigerator and place them in a centrifuge tube filled with 10mL of normal saline, vortex and oscillate to mix well; make ten-fold gradient dilutions, and spread feces suspensions with different dilutions on SL culture Then put it in an anaerobic workstation and culture it anaerobically at 37°C for about 48 hours until a single colony grows; pick single colonies with different shapes from the petri dishes that grow single colonies, and culture them on the MRS solid Purification was carried out in the base for three time...

Embodiment 2

[0077] Example 2 The biological characteristics and in vitro probiotic characteristics of Lactobacillus amylovora SLZX20-1

[0078] 1. Growth curve and determination of acid production performance:

[0079] Streak twice on the strain plate for activation, pick a single colony in MRS medium, and culture overnight at 37°C under anaerobic conditions. Then inoculate the bacterial solution into the MRS liquid medium at an inoculum amount of 1%, place it in an anaerobic workstation for anaerobic cultivation at 37°C, draw the bacterial solution every two hours, and measure the OD of the bacterial solution 600nm , the number of viable bacteria and the pH value of the bacterial solution until it enters the plateau period. Growth curve such as Figure 5 As shown, the culture time is taken as the abscissa, and the absorbance value is taken as the ordinate. The strain enters the logarithmic growth phase at 2 hours, and enters the stable growth phase at 18 hours, indicating that the str...

Embodiment 3

[0119] Example 3 Effect of Lactobacillus amylovora SLZX20-1 on growth performance and intestinal health of mice

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Abstract

The invention relates to the technical field of probiotics, in particular to lactobacillus amylovorus and application thereof. The lactobacillus amylovorus SLZX20-1 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.20122. The lactobacillus amylovous SLZX20-1 has better acid resistance and cholate resistance, strong lactic acid production capacity and better adhesion to intestinal epithelial cells, and has the capabilities of resisting pathogenic bacteria infection, promoting expression of host defense peptides of the intestinal epithelial cells, producing various enzymes such as alpha-galactosidase, beta-glucosidase, feruloyl esterase and the like. The lactobacillus amylovorus can promote intestinal development when being fed to animals, has the potential of improving the disease resistance of the animals and the feed conversion rate, and is wide in application prospect as an antibiotic substitute.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a lactobacillus amylovora and an application thereof. Background technique [0002] Antibiotics have always been used in modern pig farming. In addition to treating diseases, antibiotics are widely used in the feed industry to increase animal growth, prevent diseases, and improve growth performance. The abuse of antibiotics in feed has caused the development of drug resistance of pathogenic bacteria, antibiotic residues in animal products, environmental pollution and other disadvantages that threaten human health. However, under intensive and large-scale animal husbandry conditions, weaned piglets are vulnerable to pathogenic bacteria due to factors such as environmental factors and their own imperfect development. Therefore, it is an urgent task for the livestock industry to find green and safe alternatives to antibiotics that can promote growth and prevent diseases. Probio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/00A23K10/18A23K10/12A61K35/747A61P1/00A61P37/04A61P43/00C12R1/225
CPCC12N1/20C12N9/00A23K10/18A23K10/12A61K35/747A61P1/00A61P37/04A61P43/00A23V2400/115Y02P60/87
Inventor 马曦赵莹吴建民林姿伸张泽宇
Owner CHINA AGRI UNIV
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