Unlock instant, AI-driven research and patent intelligence for your innovation.
Liver organoid culture chip as well as preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A culture method and organoid technology, which is applied in the field of liver organoid culture chip and its preparation, can solve the problems of easy change of relative position, low throughput, complicated culture process, etc., and achieve good optical permeability and biocompatibility Good, highly user-friendly results
Pending Publication Date: 2021-11-19
合肥燃音生物科技有限公司
View PDF4 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Liver organoids obtained by matrigel culture method show great heterogeneity in shape, size, and cell composition, which seriously affects the quality controllability of liver organoid culture
In addition, during the process of culturing in Matrigel, the relative position between liver organoids is prone to change, making it difficult to locate and observe.
The above limitations lead to the limitations of the culture process of liver organoids, such as complexity, great variability, low throughput, and difficulty in real-time monitoring.
[0004] Therefore, in order to overcome the technical problems of the above-mentioned traditional liver organoid culture process, such as complexity, large variability, low throughput, and difficulty in real-time monitoring, it is necessary to develop a high-throughput in situ cultured liver organoid culture chip for liver Applied research on organoids
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0065] According to another typical implementation of the embodiments of the present invention, a method for preparing the liver organoid culture chip is provided, the method comprising:
[0066] Step S1, making on PMMA to obtain a PMMA negative mold with a microhole array;
[0069] (1) Use AutoCAD 2018 software to design the microwell array. The microwell unit is a circle with a diameter of 0.5mm and the spacing is 0.2mm. The array units are distributed in the shape of a regular hexagon (including the center point); the graphic design diagram of the microwell array is as follows figure 1 .
[0070] In the embodiment of the present invention, the regular hexagonal shape of the array unit is chosen to improve the uniformity of the spatial distribution of the array.
[0071] (2) Save the microhole array diagram designed by AutoCAD 2018 in AutoCAD R12 / LT2 DXF (*.dxf) format.
[0072...
Embodiment 1
[0083] Embodiment 1. A liver organoid culture chip and its preparation method
[0084] 1. A liver organoid culture chip, such as figure 1 shown, including:
[0086] A biological material 2 with a microwell array is arranged in the cell culture plate 1; wherein, the biological material 2 includes at least one of agarose, polyethylene glycol and sodium alginate; the biological material 2 is provided with There are a plurality of microholes 21, and the plurality of microholes 21 are uniformly arranged to form the microwell array.
[0087] The depth of each microhole is 1mm. The shape of the hole on the microhole is circular.
[0088] The distance between two adjacent micropores is 0.2mm.
[0089] The area of each micropore is 0.19625mm 2 .
[0090] The biological material is agarose with a concentration of 2%.
[0091] 2. The preparation method of the liver organoid culture chip
[0110] In the embodiment of the present invention, the depth of each microhole is 0.1mm. The distance between two adjacent micropores is 0.01 mm. The area of each micropore is 78.5×10 -6 mm 2 . The biological material is agarose at a concentration of 0.2%. Other structures and steps are all with embodiment 1.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
depth
aaaaa
aaaaa
area
aaaaa
aaaaa
depth
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a liver organoid culture chip as well as a preparation method and an application thereof. The liver organoid culture chip comprises a cell culture plate, a culture medium and a culture medium, the biological material is provided with a micropore array and is arranged in the cell culture plate; wherein the biological material comprises at least one of agarose, polyethylene glycol and sodium alginate; the biological material is provided with a plurality of micropores, and the plurality of micropores are uniformly arranged to form the micropore array. The culture method comprises the following steps: digesting human embryonic stem cells or human induced multifunctional stem cells into single cells, inoculating the single cells into a culture medium, and culturing to obtain preintestinal embryonic cells; and digesting the preintestinal embryo cells into single cells, inoculating the single cells into the micropore array in the liver organoid culture chip, and culturing to obtain the liver organoid. The method is suitable for culturing uniform and high-throughput liver organoids with different sources or different tissue types.
Description
technical field [0001] The invention relates to the technical fields of tissue engineering and organ chips, in particular to a liver organoid culture chip and its preparation method and application. Background technique [0002] Organoids are collections of organ-specific cells that develop from stem cells or organ progenitor cells and are capable of self-organization by cell sorting and spatially restricted lineage differentiation in a manner similar to that in humans. In short, organoids are a three-dimensional (3D) in vitrocell culturesystem, which can replicate the complex spatial morphology of differentiated tissues, and can express the relationship between cells and between cells and their surrounding matrix. Interactions and spatial location patterns. Organoids can have similar physiological responses to differentiated tissues in the human body, and have a high similarity to human-derived tissues. Compared with the traditional two-dimensional (2D) cell culture mod...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More