Method for screening antioxidant by using phascolosoma esculenta

Abstract

The invention discloses a method for screening an antioxidant by using phascolosoma esculenta, which comprises three steps of phascolosoma esculenta culture, phascolosoma esculenta ROS model establishment and model verification. The establishment of the phascolosoma esculenta ROS model comprises the following six steps: selecting tested phascolosoma esculenta, determining lymphatic blood cells for producing ROS, determining a clear cell separation method, determining the concentration and action time of a positive control compound H2O2, and performing ROS in-vitro stability and reproducibility experiments. The phascolosoma esculenta model is used for screening the antioxidant and has a good application prospect, and the phascolosoma esculenta belongs to closed-tube circulating body cavity animals and can truly reflect organism metabolism; transparent cells of phascolosoma esculenta are thin in membrane, and fluorescent markers and antioxidants are easy to permeate into the transparent cells, so that an anti-oxidation result can be better displayed; the phascolosoma esculenta is easy to breed, the cost is low, and the experimental operation is simple.

Description

technical field [0001] The invention belongs to the technical field of screening antioxidants, and in particular relates to a method for screening antioxidants by using D. pallidum. Background technique [0002] With the improvement of living standards, people pay more attention to their health. Active oxygen is an important substance that affects biological signal transduction, and its accumulation is associated with the occurrence of various chronic diseases such as aging and cardiovascular disease. The antioxidant activity of many pure compounds, foods, and dietary supplements has been extensively studied, and screening for new antioxidants (ie, free radical scavengers) is ongoing. [0003] At present, antioxidant screening evaluation models mainly include in vitro evaluation and in vivo evaluation methods, of which in vitro evaluation mainly includes chemical evaluation method and cell model evaluation method, and in vivo evaluation methods mainly include animal evaluat...

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Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a method for screening antioxidants by using D. coli, which solves the problem that the chemical evaluation method cannot truly simulate the physiological environment, does not consider the drug transmembrane into cells, etc., cell model evaluation The method does not take into account the metabolism in the body, etc., and the animal model method of rats and mice is expensive, time-consuming, and difficult to operate.

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