Production method of modified oviductus ranae
A production method and technology of Rana oil, applied in the direction of fat oil/fat production, fat production, animal husbandry, etc., can solve the problems of restricting the consumption and promotion of Rana oil, accelerated tumor proliferation, and unsustainable, to achieve Facilitate digestion and absorption, ensure continuous utilization, and prolong the effect of utilization cycle
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Embodiment 1
[0032] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:
[0033] (1) spawning in clean water:
[0034] In the Changbai Mountain area of China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;
[0035] (2) Treatment of fertilized eggs:
[0036] When the paired female frogs discharge the egg mass, take it out for 10 minutes, wash it, and place it on a growth plate at 20°C for 9 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly put them into a -18°C freezer for freezing;
[0037] (3) Freeze drying:
[0038] Put the frozen frog embryos into a freeze dryer for d...
Embodiment 2
[0042] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:
[0043] (1) spawning in clean water:
[0044] In the Changbai Mountain area of China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;
[0045] (2) Treatment of fertilized eggs:
[0046]When the eggs are discharged from the female frog, they are taken out and cleaned for 12 minutes, and placed on a growth plate at 22°C for 8.5 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly enter the freezer at -18°C for cryopreservation;
[0047] (3) Freeze drying:
[0048] Put the frozen frog embryos into a freeze...
Embodiment 3
[0052] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:
[0053] (1) spawning in clean water:
[0054] In the Changbai Mountain area of China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;
[0055] (2) Treatment of fertilized eggs:
[0056] When the eggs are released from the female frog, take them out for 15 minutes, wash them, and place them on a growth plate at 24°C for 8 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly put them into a -18°C freezer for freezing;
[0057] (3) Freeze drying:
[0058] Put the frozen frog embryos into a freeze dryer for...
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