Production method of modified oviductus ranae

A production method and technology of Rana oil, applied in the direction of fat oil/fat production, fat production, animal husbandry, etc., can solve the problems of restricting the consumption and promotion of Rana oil, accelerated tumor proliferation, and unsustainable, to achieve Facilitate digestion and absorption, ensure continuous utilization, and prolong the effect of utilization cycle

Pending Publication Date: 2021-11-26
吉林省蛙王生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sex hormones function through corresponding receptors, and have targeted affinity for some receptors, which is manifested as stimulation and affinity for some existing tumor cells, resulting in accelerated tumor proliferation, which also limits The consumption and promotion of Rana oil has had a direct impact on

Method used

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  • Production method of modified oviductus ranae
  • Production method of modified oviductus ranae
  • Production method of modified oviductus ranae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:

[0033] (1) spawning in clean water:

[0034] In the Changbai Mountain area of ​​China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;

[0035] (2) Treatment of fertilized eggs:

[0036] When the paired female frogs discharge the egg mass, take it out for 10 minutes, wash it, and place it on a growth plate at 20°C for 9 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly put them into a -18°C freezer for freezing;

[0037] (3) Freeze drying:

[0038] Put the frozen frog embryos into a freeze dryer for d...

Embodiment 2

[0042] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:

[0043] (1) spawning in clean water:

[0044] In the Changbai Mountain area of ​​China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;

[0045] (2) Treatment of fertilized eggs:

[0046]When the eggs are discharged from the female frog, they are taken out and cleaned for 12 minutes, and placed on a growth plate at 22°C for 8.5 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly enter the freezer at -18°C for cryopreservation;

[0047] (3) Freeze drying:

[0048] Put the frozen frog embryos into a freeze...

Embodiment 3

[0052] The invention provides a kind of production method of modified wood frog oil, comprises the following steps:

[0053] (1) spawning in clean water:

[0054] In the Changbai Mountain area of ​​China, from April to May every year, the forest frogs are caught in the pre-breeding stage after the stinging. The female and male frogs enter their respective temporary storage pools for pre-heating. The temperature of the pool water is 15-20°C. According to the ratio of male to female ratio of 1:1.5, they are mixed into the clean water pool to hold pairs and lay eggs;

[0055] (2) Treatment of fertilized eggs:

[0056] When the eggs are released from the female frog, take them out for 15 minutes, wash them, and place them on a growth plate at 24°C for 8 hours, so that the fertilized eggs develop to the end of the blastocyst stage, and then quickly put them into a -18°C freezer for freezing;

[0057] (3) Freeze drying:

[0058] Put the frozen frog embryos into a freeze dryer for...

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Abstract

The invention provides a production method of modified oviductus ranae, which comprises the steps of oviposition in purified water, fertilized egg treatment, freeze drying, oil-egg separation and the like, and the obtained oviductus ranae is the modified oviductus ranae. According to the method, pure and impurity-free oviductus ranae without sex hormones is extracted under the condition of not needing excessive external intervention according to the embryonic development characteristics of Rana amurensis, and the obtained oviductus ranae does not have the characteristics of form, component content, medicine property and the like of traditional oviductus ranae, but is similar to the traditional oviductus ranae in water absorption expansion rate and smell. The protein content is inversely proportional to the time before the embryonic development is completed, and the contents of polypeptides, oligopeptides and amino acids are directly proportional to the time before the embryonic development is completed. The method does not damage the health and normal oviposition and survival of the wood frogs, prolongs the utilization cycle of the wood frogs, upgrades the traditional oil extraction by killing the frogs into oil extraction by raising the frogs, and has important significance for protecting the precious germplasm resource of the wood frogs in China, orderly developing and reasonably utilizing the wood frogs and benefiting the society.

Description

technical field [0001] The invention relates to a production method of wood frog oil, in particular to a production method of modified wood frog oil. Background technique [0002] Rana oil is a traditional medicine and health food in my country. It has various medicinal and health effects such as anti-fatigue and regulating human endocrine (Gaofeng et al., 2005). The main component of Rana oil is protein. In the 1930s, Japanese scholars analyzed its components and found that the protein content accounts for more than 50% of the total amount of Rana oil. Rana oil has a complete range of amino acids and is rich in content. At the same time, it contains a variety of unsaturated fatty acids and esters, and is also rich in vitamins and trace elements (Han Tiesuo et al., 2008). [0003] The most well-known medicinal effect of Rana oil is "nourishing yin", which means it has a special nourishing effect on women. Many domestic scholars have therefore also detected the sex steroid...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/02A01K67/02
CPCC11B1/00C11B1/02A01K67/02
Inventor 房永清刘志文
Owner 吉林省蛙王生物工程有限公司
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