SNP molecular marker related to pig backfat thickness and application thereof

A technology of molecular markers and pig backfat thickness, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of large distance span and limited application, so as to reduce feed intake and speed up the breeding process , the effect of increasing the frequency

Pending Publication Date: 2021-11-26
SOUTH CHINA AGRI UNIV
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Problems solved by technology

However, due to the randomness of the selection of candidate genes in the candidate gene method, they may be the main gene, or the gene that is in close linkage disequilibrium with the actual QTL and indirectly affects the traits, which brings new problems to livestock breeding. In addition, the selection of the candidate gene method also has population heterogeneity; while the QTL genetic distance obtained by QTL mapping has a large span, often including hundreds of genes, which greatly limits the application of QTL mapping methods in important economic traits of livestock. Applications in Genetic Improvement

Method used

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  • SNP molecular marker related to pig backfat thickness and application thereof
  • SNP molecular marker related to pig backfat thickness and application thereof
  • SNP molecular marker related to pig backfat thickness and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] (1) Experimental animals

[0030] The experimental pig group used in the present invention is the purebred American Duroc head of the Breeding Pig Branch of Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.

[0031] In this experiment, a total of 3769 Duroc pigs were selected in this resource group. The pigs in this experiment had free access to food and water, and the entire feeding method and feeding conditions were always consistent, which is a conventional method.

[0032] Duroc pigs have a small number of litters, with an average of only 9 to 10 pigs in a large group, but they grow fast, have a high feed conversion rate, a high carcass lean meat rate, a high intramuscular fat content, and strong stress resistance. Domestic commercial pig production has established a relatively mature hybrid supporting system. Among them, the hybrid combinations of Du Dachang continue to maintain an absolute dominant position in my country's domestic a...

Embodiment 2

[0052] Example 2 Target DNA sequence amplification and sequencing

[0053] (1) Primer design

[0054] The DNA sequence of SEQ ID NO: 1 on pig chromosome 1 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers, entrust Sangon Bioengineering (Shanghai) Co., Ltd. to synthesize primers. The DNA sequences of the designed primers are as follows:

[0055] P001-F: 5'-ATTGGTGCTTTTCCTTAGAAGTCTTTC-3',

[0056] P002-R: 5'-GTCTACACATCCAGGCTTTCTCC-3';

[0057] (2) PCR amplification

[0058] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 64.5°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0059] (3) DNA sequenc...

Embodiment 3

[0063] Example 3 SNP site g.135A>G effect analysis of molecular markers

[0064] According to Table 1 and figure 2 It can be seen that for the backfat thickness, the effect of the SNP site g.135A>G dominant allele (AA) significantly reduces the backfat thickness by 1.16 mm on average than the GG phenotype. The phenotypic correlation analysis results of growth rate and backfat thickness traits show that the age at 100kg body weight is positively correlated with backfat thickness, reaching a very significant level, that is to say, the younger the pig grows to 100kg body weight, the more backfat. The thinner the meat, the higher the lean meat rate, which will greatly increase the economic value of pork and create wealth for the enterprise. In the individuals with this SNP molecular marker, by optimizing the dominant allele (A) of the SNP in American Duroc, the economic benefits of commercial pigs can be finally improved, thereby increasing the income of the enterprise.

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to pig backfat thickness and application thereof, the locus of the SNP molecular marker is the 38161769th nucleotide locus on a chromosome 1 of an international pig reference genome version 11.1, and the basic group of the locus is A or G. By optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the backfat thickness of boars can be reduced, the excellent boars with the characters can be bred, and the genetic improvement progress of the boars can be accelerated, so that the economic benefit of boar breeding can be effectively improved.

Description

technical field [0001] The invention relates to a SNP molecular marker related to pig backfat thickness and its application. Background technique [0002] Pork is an important meat source for humans, accounting for about 40% of the meat consumed by the world's population. Affected by Chinese meat consumption habits, my country's demand for pork is even greater. Since the 1980s, pork has been the main meat on the tables of urban and rural residents, and pork consumption by urban and rural residents has always accounted for more than 60% of meat consumption, occupying a dominant position. In 2020, the total number of pigs slaughtered in my country will be 527 million heads, accounting for half of the world's pigs slaughtered; pork production will be 41.13 million tons, accounting for 42.02% of global pork production, also ranking first. The huge demand for pork is mainly because it can provide a large amount of human necessary protein demand, and protein mainly comes from le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122
Inventor 杨杰吴珍芳丁荣荣郑恩琴庄站伟蔡更元李紫聪顾婷徐铮
Owner SOUTH CHINA AGRI UNIV
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