Establishment method and application of machin type 2 diabetes model

Abstract

The invention relates to the technical field of non-human primate experimental animal disease models, and discloses an establishment method and application of a machin type 2 diabetes model. The method comprises the following steps of (1) selecting male machin of which the age is 12-21 years old; (2) feeding the male machin for four times every day, specifically, feeding the male machin with sugar-containing feed for the first time, feeding the male machin with complementary food for the second time, feeding the male machin with green feed for the third time and feeding the the male machin with the sugar-containing feed for the fourth time; and (3) after feeding the male machin for one year according to the mode in the step (2), detecting corresponding indexes, and selecting the machin with the blood glucose level larger than 7 mmol / L in the fasting state, wherein the fed complementary food is brown sugar blocks and biscuits alternately, and the brown sugar blocks and the biscuits are alternated once every three days; and the sugar-containing feed is obtained by mixing conventional feed and brown sugar. According to the method, the machin type 2 diabetes model is established by adopting a warm feeding mode, and the machin type 2 diabetes model can be used for drug effect evaluation in the research and development process of new drugs.

Description

technical field [0001] The invention relates to the technical field of non-human primate experimental animal disease models, in particular to a method for establishing a cynomolgus monkey type 2 diabetes model and its application. Background technique [0002] Diabetes is a metabolic disease characterized by high blood sugar. Long-term high blood sugar can lead to chronic damage and dysfunction of various tissues, especially eyes, kidneys, heart, blood vessels, and nerves. Acute severe metabolic disorders, such as diabetic ketoacidosis and hyperosmolar hyperglycemia syndrome, can occur in severe illness or stress. In my country, more than 90% of the patients are type 2 diabetes. Type 2 diabetes mellitus is a metabolic disease caused by the combination of two main causes, the defective insulin secretion of pancreatic β cells and the failure of insulin response in insulin-sensitive tissues (such as: skeletal muscle, cardiac muscle and adipose tissue). According to the Inter...

AI Technical Summary

Problems solved by technology

However, non-human primates hate high-fat diets and are prone to diarrhea, rectal prolapse and other diseases

Also, a diet high in saturated fat and cholesterol inhibits endogenous cholesterol synthesis, limiting the model's utility

The use of aging spontaneous type 2 diabetes models is limited due to the long period and high cost

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