Geobacterium galactose BWTGW1.1 and application thereof

A galactose and geological technology, applied in the field of environmental microorganisms, can solve the problems of harmlessness and low fertility, long time-consuming natural fermentation, serious odor pollution, etc., achieve great application potential, promote the fermentation process and the duration of the high-temperature stage , the effect of strong growth adaptability

Pending Publication Date: 2021-11-30
广东博沃特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional composting method mainly uses the fermentation of indigenous microorganisms in organic waste, which ha

Method used

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  • Geobacterium galactose BWTGW1.1 and application thereof
  • Geobacterium galactose BWTGW1.1 and application thereof
  • Geobacterium galactose BWTGW1.1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1 Purification and preservation of galactose geological embodiment Bacillus BWTGW1.1

[0030] March 2020 from Dongguan city center designated slaughterhouses Co., mud pig manure composting organic waste fermentation stage high temperature (50 ℃) collected three middle samples.

[0031] Isolation and purification of high-temperature storage: 3 samples collected by each said 10g 50mL PBS (pH 7.4, concentration of 0.2mol / L) The suspension, shaken vigorously 5min, 1000r / min centrifugation 3 times, each time 5min, the supernatant was collected to a centrifuge tube. The supernatant was diluted at different concentrations, take 10 -3 10 -4 10 -5 On LB agar plates coated with bacteria gradient, 60 ℃ incubated 48h. Picked colonies grown larger strain is preferably continued streaked on LB agar plates, repeatedly purified.

[0032] The colonies were purified cells placed in 25% glycerol dispersion tube, -80 ℃ stored for use. Thus, the strain BWTGW1.1.

Example Embodiment

[0033] Example 2 Identification of 16S rDNA galactose geology of Bacillus BWTGW1.1

[0034] (1) morphological analysis: The strain is applied to BWTGW1.1 screened on an LB plate, 60 deg.] C for 24h after which colony characteristics were observed; single colony smear, Gram stain, cell morphology was observed under a microscope The results see figure 1 .

[0035] (2) 16S rRNA gene Molecular identification:

[0036]The genome DNA of strain BWTGW1.1 was extracted, and the PCR product was amplified by bacterial 16S RDNA gene amplification general primer 27F / 1492R (5'-agagttgatcctccaatc-3 'and 5'-tacgacttaAccccaatccc-3') obtained by PCR product, and sent to Shanghai Meiji Biomine Medicine Technology Co., Ltd. (Guangzhou Branch) performs sequence sequencing, and the 16S rRNA sequence obtained after sequencing is 1447 bp, and sequence is shown in SEQ ID NO.1. The sequencing results were homologous comparison analysis with the 16SRDNA sequences in the NCBI and EZBiocloud database, then ...

Example Embodiment

[0039] Example 3 High-temperature protease capability of high temperature protease BWTGW1.1 in galactose Gobacterium

[0040] High-temperature protease capacity evaluation using plate water release ring detection:

[0041] The main use of the medium and reagents are as follows:

[0042] Protein medium: 40.0.0g, soluble starch 10.0g, yeast paste 3.0g, potato powder 2.0g, agar 15.0 g, add distilled water to 1000 mL, pH 7.0 ~ 7.2.121 ° C high temperature high pressure sterilization 20 min, pour flat plate .

[0043] After the activated galactose bobacillus BWTGW1.1 is connected to the protein medium plate, after culturing 48 h in a 40 ° C incubator, it is observed whether or not the hydrolyzed transparent circle surrounding the colonies is generated, and the customity directly reflects. Whether the strain is secreted to produce high temperature protease.

[0044] The above-described method was observed to obtain a hydrolyzed transparent circle in the high temperature of the galactose...

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Abstract

The invention discloses a geobacterium galactose BWTGW1.1 and an application thereof. Geobacillus galactosidassius BWTGW1.1 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on November 11, 2020, the address of the GDMCC is the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC No. 61374. The Geobacterium galactosidasius BWTGW1.1 disclosed by the invention is strong in growth adaptability in high-temperature compost of pig manure sludge wastes, is active in a high-temperature stage, can generate high-temperature-resistant protease, and has a relatively great application potential in aerobic compost of livestock and poultry breeding organic wastes.

Description

Technical field: [0001] The invention relates to the technical field of environmental microorganisms, in particular to a Geobacter galactosus BWTGW1.1 and its application in the treatment of solid organic waste from livestock and poultry breeding. Background technique: [0002] In recent years, domestic and foreign experience in the treatment of aquaculture organic waste has shown that high-temperature aerobic composting of aquaculture waste to produce high-quality organic fertilizer is an important way to recycle this type of waste. In the process of aerobic composting, on the one hand, the biodegradable high-molecular organic matter is decomposed by the rapid reproduction of microorganisms, and a new stable high-molecular organic matter --- humus (an important active factor that constitutes soil fertility) is synthesized; on the other hand The massive reproduction of microorganisms releases heat, which makes the heap continue to heat up in a short period of time, thereby k...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/52C05F3/00C05F17/20C12R1/01
CPCC12N9/52C05F3/00C05F17/20Y02W30/40Y02A40/20
Inventor 陈猛谢小林周莲李成江刘玉敏朱红惠陈美标
Owner 广东博沃特生物技术有限公司
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