Strain capable of highly producing vitamin K2 (MK-7), screening method of strain and method for producing vitamin K2 (MK-7)
A high-yield strain, MK-7 technology, applied in the field of microbial fermentation, can solve the problems of not meeting the normal intake of the human body, low activity cis isomers, complex reaction steps, etc., achieve good passaging stability, and reduce pollution emissions , the effect of increasing production
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0033] Screening of vitamin K2 (MK-7)
[0034] 1.1, preparation of the medium
[0035]LB medium preparation: 5 g of yeast extract, 10 g of tryptone, 5 g sodium chloride, 15 g of agar, is disposed to 1000 mL with distilled water, and then the pH is adjusted to 7.0 ± 0.2 with a mass fraction of 30% sodium hydroxide solution. In several test tubes, 20 min at 121 ° C is sterilized. After sterilization is completed, a slope medium is obtained, and a slope medium is obtained, and the sterilized Lb medium is poured into the plate to obtain an Lb medium.
[0036] Seed medium preparation: 10 g of glycerol, 5g yeast, 10g soy protein, distilled water to 1000 mL, then adjust pH to 7.0 ± 0.2 with a mass fraction of 30% sodium hydroxide solution, each 250ml triangle bottle 30 ml seed medium, 8-slice gauze seal, 20 min at 121 ° C for 20 min, and the seed medium was obtained.
[0037] Fermentation medium preparation: 180 g of glycerol, 15g yeast powder, 240 g soy protein, 7.5 g sodium chloride, 3...
Embodiment 2
[0045] Study on Genetic Stability of Vitamin K2 (MK-7)
[0046] (1) Paste atrium BK21018 prepared in Example 1 was inoculated in Example 1 in the case of the beveled medium prepared in Example 1, and the beveled strain was deeply accumulated under 37 ± 0.5 ° C, relative humidity 40% -60%. After mature, it is again cultured in a beveled medium and repeated four times;
[0047] (2) The five-cultured strains in step (1) each transferred to the seed medium prepared in Example 1, cultured at 37 ± 0.5 ° C, relative humidity 40% -60%, 220 rpm, 16 When the bacteria was ripe, each of the fermentation medium prepared by the preparation Example 1 was taken in 37 ± 0.5 ° C, the relative humidity was 40% -60%, 220 rpm, and the five groups contain vitamin K2 ( MK-7) fermentation liquid;
[0048] (3) The effect of vitamin K2 (MK-7) in the fermentation liquid in the fermentation liquid in HPLC, and the result is: 232 g / ml, 239 g / ml, 231 g / ml, 236 g / ml according to the sequence of culture....
Embodiment 3
[0051] Vitamin K2 (MK-7) high-strain fermentation process control
[0052] 3.1, Preparation of Medium
[0053] Seed tank medium preparation: 100g glycerol, 100g yeast powder, 100g soy protein, to 9L with tap water, then adjust pH to 7.0 ± 0.2 with a hydroxide solution of mass fraction of 30%, and carry out seed can For dismissal, sterilization of 30 min at 121 ° C, cooled by circulating water, and 10 L seed culture is obtained.
[0054] Fermentation tank medium preparation: 3000 g of glycerol, 250g yeast powder, 4000 g soy protein, 150 g sodium chloride, 500g sucrose, 1 g nicotinamide, 1 g aspartic acid, use tap water to 45L, then use mass fraction of 30 The% sodium hydroxide solution regulates pH to 7.0 ± 0.2, and the fermenter was eliminated, 30 min at 121 ° C, cooled by circulating water, and 50 L of fermentation medium was obtained.
[0055] Fermentation process control of high strains: BK21018 strain inoculated into the LB medium, and in 37 ± 0.5 ° C, relative humidity 40% -6...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com