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Nucleic acid composition, kit and method for detecting medication-related genes of mental diseases by matrix-assisted laser desorptiontime of flight mass spectrometry

A technology of time-of-flight mass spectrometry and matrix-assisted laser, which is applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of high price, high technical personnel requirements, and low throughput, and achieve low-cost effects

Pending Publication Date: 2021-12-07
厦门市仙岳医院(厦门市精神卫生中心)
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Problems solved by technology

However, the above-mentioned methods all have disadvantages such as low throughput, few detection sites, and high price. In particular, the ARMS method is difficult to design primers and requires high technical personnel.

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  • Nucleic acid composition, kit and method for detecting medication-related genes of mental diseases by matrix-assisted laser desorptiontime of flight mass spectrometry
  • Nucleic acid composition, kit and method for detecting medication-related genes of mental diseases by matrix-assisted laser desorptiontime of flight mass spectrometry
  • Nucleic acid composition, kit and method for detecting medication-related genes of mental diseases by matrix-assisted laser desorptiontime of flight mass spectrometry

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Embodiment Construction

[0020] The present invention is further described below in conjunction with embodiment.

[0021] This application adopts EDTA anticoagulant blood sample, extracts DNA with automatic nucleic acid extractor, realizes amplification, SAP reaction and extension reaction with PCR instrument, transfers to detection chip after desalting the obtained analysis product, utilizes MassARRAY system (including MassARRAY Analyzer and An integrated liquid handler known as the chip preparation module (CPM) performs detection on the chip and enables interpretation of the results.

[0022] Further, the detection method adopted in this application is multiplex PCR combined with nucleic acid flight mass spectrometry. Divide the 42 target sequences into two systems (the first well and the second well), realize simultaneous amplification of the 42 target sequences in the two systems by multiplex PCR, and then add specific extension primers for each SNP sequence, Extend 1 base at the SNP site. After...

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Abstract

The invention discloses a nucleic acid composition, a kit and a method for detecting medication-related genes of mental diseases through laser desorptiontime of flight mass spectrometry. The nucleic acid composition comprises an upstream primer and a downstream primer of 42 SNP (Single Nucleotide Polymorphism) loci, and nucleic acid sequences are as shown in SEQ ID NO.1-84. The application covers genes related to pharmacokinetics, pharmacodynamics and (or) severe adverse reactions of drugs, and the loci are selected according to authoritative literature reports and guidelines; primer design and detection are carried out on the 42 SNP loci of 30 genes related to medication of mental diseases including antipsychotic drugs, antidepressant drugs, antianxiety drugs, sedative-hypnotic drugs and antiepileptic drugs, and guidance can be provided for precise medication of the diseases. Compared with the prior art, the method has the advantages of rapidness, sensitivity, high throughput, low cost and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid composition, a kit and a method for detecting genes related to medication for mental diseases by matrix-assisted laser desorption time-of-flight mass spectrometry. Background technique [0002] Mental illness has become a high incidence disease in our country. According to statistics, more than 17% of adults in our country suffer from depression, schizophrenia and other mental illnesses. Mental illnesses mainly refer to diseases that are clinically manifested by disorders of brain function under the influence of various biological, psychological, and social environmental factors, leading to varying degrees of impairment in mental activities such as cognition, emotion, will, and behavior. It is mainly divided into mild mental illness and severe mental illness. Common mild mental illnesses include depression, anxiety, obsessive-compulsive disorder, etc., and common sever...

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686
CPCC12Q1/6883C12Q1/686C12Q2600/156C12Q2600/106C12Q2537/143C12Q2565/627C12Q2533/101
Inventor 徐象珍丁丽君陈彬彬黄颖华吕海燕马春玲刘发荣
Owner 厦门市仙岳医院(厦门市精神卫生中心)