Method for obtaining filial generation of cultivated tobacco and nicotiana alata
A technology for cultivating tobacco and flowering tobacco is applied in the field of obtaining hybrid progeny of cultivated tobacco and flowering tobacco, which can solve the problem that the affinity between cultivated tobacco and flowering tobacco is not significantly improved, and achieves ensuring possibility, improving affinity, and avoiding interference. Effect
Active Publication Date: 2021-12-14
中国烟草总公司贵州省公司
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Problems solved by technology
[0005] The main purpose of the present invention is to provide a method for obtaining the hybrid offspring of cultivated tobacco and Nicotiana tabacum, aiming to solve the technical problem that the affinity between cultivated tobacco and Nicotiana tabacum has not been significantly improved in the prior art
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Embodiment 1
[0064] 1. Take the mature pollen of the first cultivated tobacco, and then use the mature pollen of the first cultivated tobacco to carry out the in vivo induction treatment for 1 h on the stigma of the second cultivated tobacco to obtain the stigma after the first treatment ; After the induction treatment, the stigma after the first treatment contains an induction system that can enhance the hybridization affinity.
[0065] 2. Immediately after the induction treatment, the stigma treated for the first time is subjected to cell crushing, drying (drying at room temperature) and crushing treatment in turn to obtain the stigma after the second treatment .
[0066] 3. Take the pollen of Nicotiana tabacum, and mix the mature pollen of Nicotiana tabacum with the stigma after the second treatment at a mass ratio of 1:10 to obtain a mixture to be treated, which contains the cultivated Tobacco induction system, which can significantly improve the affinity of hybridization.
[0067] 4...
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The invention provides a method for obtaining filial generation of cultivated tobacco and nicotiana alata. The method comprises the following steps: taking pollen of first cultivated tobacco, and then performing induction treatment on stigmas of second cultivated tobacco by using the pollen of the first cultivated tobacco to obtain stigmas subjected to first treatment; sequentially carrying out cell disruption treatment, drying treatment and mashing treatment on the stigmas subjected to the first treatment to obtain stigmas subjected to second treatment; taking pollen of the nicotiana alata, and mixing the pollen of the nicotiana alata with the stigmas subjected to second treatment according to a preset proportion to obtain a mixture to be treated; repeatedly pollinating the third cultivated tobacco for multiple times within 120 hours after the initial opening period through the to-be-treated mixture to obtain the pollinated cultivated tobacco; and carrying out detection operation and screening operation on the pollinated cultivated tobacco and the obtained filial generation to obtain the tobacco hybrid variety with stress resistance. According to the method, the affinity of the cultivated tobacco and the nicotiana alata parent can be remarkably improved, and about 5%-8% of real filial generations can be obtained.
Description
technical field [0001] The invention relates to the technical field of tobacco hybridization, in particular to a method for obtaining hybrid offspring of cultivated tobacco and Nicotiana tabacum. Background technique [0002] Nicotiana nicotiana (N.alata, n=9) of the subgenus Petunia has strong disease resistance, and has good resistance to wildfire disease, powdery mildew and tomato spot disease. In existing studies, conventional pollination methods have been explored to try to introduce the excellent traits of N.alata into cultivated tobacco species, but no good results have been obtained, and the existing research has not found the time, location and breakthrough of hybrid incompatibility. Methods of hybridization incompatibility have not been systematically studied. [0003] In order to solve the above problems, the Chinese invention patent with the publication number CN104996295A discloses a method for improving the hybrid affinity and seeding rate of cultivated tobacc...
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Login to View More IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/021A01H4/008
Inventor 吕立堂陈穗云廖菊够
Owner 中国烟草总公司贵州省公司