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Crape myrtle salt-tolerant gene LiCIPK24 and application thereof

A technology of amino acid and protein, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of staining pollution, large amount of physical improvement engineering, high cost, etc.

Active Publication Date: 2021-12-14
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly three types of saline-alkali land treatment: physical improvement, chemical improvement and biological improvement. Among them, physical improvement requires a large amount of engineering and high cost; chemical improvement has the potential to cause secondary salinization pollution.

Method used

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  • Crape myrtle salt-tolerant gene LiCIPK24 and application thereof
  • Crape myrtle salt-tolerant gene LiCIPK24 and application thereof
  • Crape myrtle salt-tolerant gene LiCIPK24 and application thereof

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Effect test

Embodiment 1

[0079] 1. cDNA clone of Lagerstroemia indica salt tolerance gene LiCIPK24

[0080] The total RNA of the aerial parts of annual Lagerstroemia crape myrtle seedlings was extracted with the plant RNA mini-extraction kit (TaKaRa), and the first-strand cDNA was obtained with the cDNA reverse transcription kit (TAKARA). Through homologous comparison, the homologous gene sequences of CIPK24 of Eucalyptus and Arabidopsis were obtained, primers F1 and R1 of homologous sequences were designed with Oligo7, 3'RACE primers F2 and F3 were designed according to the obtained sequence of segment A, and Refer to the 3'RACE method of the SMARTer™ RACE cDNA Amplification Kit (Clontech, USA) to amplify, and obtain the 3' end sequence B, fragment A and fragment B are spliced ​​to obtain the full-length sequence of the LiCIPK24 gene (SEQ ID NO .1, the length is 1552bp. According to Seq. NO. 1, primer R2 was designed, paired with F1 for amplification, and the sequence (1335bp) containing SEQ ID NO.2 ...

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Abstract

The invention discloses a crape myrtle salt-tolerant gene LiCIPK24 and application thereof, and belongs to the technical field of biology. The amino acid sequence of the protein coded by the lagerstroemia indica salt-tolerant gene LiCIPK24 is shown as SEQ ID NO.3. According to the invention, a crape myrtle CIPK member is obtained for the first time, and the crape myrtle CIPK member is overexpressed in arabidopsis thaliana to find the biological function of the crape myrtle CIPK member in the aspects of salt stress resistance and osmotic stress resistance of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a crape myrtle salt-tolerant gene LiCIPK24 and an application thereof. Background technique [0002] Lagerstroemia indica L. Alias: itchy flower, itchy tree, purple golden flower, barkless tree. Crape myrtle is native to Southeast Asia to Oceania. Wild species grow on hillsides or forest margins below an altitude of 1200m. It is widely planted in tropical regions. It is a deciduous shrub or small tree of the genus Lagerstroemia in dicotyledonous plants, and can reach a height of 7 rice. Xuzhou and other places have listed it as a city flower, and it is widely used in gardens. [0003] Soil salinization is one of the main problems threatening crop productivity in the world. At present, the problem of soil salinization is becoming more and more serious. The area of ​​saline-alkali land in the world is 1 billion hectares, of which my country is about 99 million hectares, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/84A01H5/00A01H6/20
CPCC12N9/12C12N15/8273C12Y207/11
Inventor 余春梅柯勇超赵燕春丁志佳刘昱刘国元陈艳红钟非魏辉张健
Owner NANTONG UNIVERSITY
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