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Inorganic Phosphorus-decomposing Bacteria in the Rhizosphere of a Poplar and Its Application

A technology for decomposing inorganic phosphorus bacteria and inorganic phosphate, which is applied in the field of agricultural microorganisms, can solve the problem of less phosphorus decomposing bacteria, achieve stable phosphorus decomposing ability, strong dissolving ability, and promote growth

Active Publication Date: 2020-08-25
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on phosphorus-solubilizing bacteria is mainly focused on agricultural development, and there are relatively few phosphorus-solubilizing bacteria screened from the rhizosphere of forest trees.

Method used

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  • Inorganic Phosphorus-decomposing Bacteria in the Rhizosphere of a Poplar and Its Application
  • Inorganic Phosphorus-decomposing Bacteria in the Rhizosphere of a Poplar and Its Application
  • Inorganic Phosphorus-decomposing Bacteria in the Rhizosphere of a Poplar and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the dissolving ability of inorganic phosphorus bacterium Mp1-Ha3 to different phosphorus sources

[0022] 1. Medium formula

[0023] LB medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, deionized water 1000mL, pH7.0.

[0024] NBRIP medium: glucose 10 g, Ca 3 (PO 4 ) 2 5 g, MgCl 2 .6H 2 O 5 g, KCl 0.2 g, MgSO 4 .7H 2 O 0.25 g, (NH 4 ) 2 SO 4 0.1 g, 1000 mL of distilled water, pH 7.0, when measuring the solubility of the strain to different phosphorus sources, the Ca in the medium formula should be 3 (PO 4 ) 2 Replaced by AlPO 4 or FePO 4 .

[0025] 2. Experimental method

[0026] The strain Mp1-Ha3 stored at -80°C was activated and transferred twice, then inoculated in LB liquid medium, and cultured at 28°C and 180 r / min for 12 h. Take 1 mL of bacterial liquid, centrifuge at 10,000 rpm for 1 min, remove the supernatant, and then wash twice with sterile distilled water to make seed liquid. Then inoculate the seed liquid i...

Embodiment 2

[0028] Example 2: Effects of Different Levels of Soluble Phosphorus Concentrations on Phosphorus Solubilizing Activity of Inorganic Phosphorus Solubilizing Bacteria Mp1-Ha3

[0029] 1. Medium

[0030] Exogenous soluble phosphorus source: 100mmol / L K 2 HPO 4 .

[0031] SP medium: glucose 10 g, (NH 4 ) 2 SO 4 0.6 g, KCl 0.4 g, Ca 3 (PO 4 ) 2 5 g, 1L deionized water, pH7.0.

[0032] 2. Experimental method

[0033] with K 2 HPO 4 (100 mmol / L) was the source of exogenous soluble phosphorus, and the SP medium with final concentrations of exogenous soluble phosphorus of 0, 0.5, 1.0, 5.0, 10.0, 20.0 and 25.0 mmol / L were prepared. Prepare the seed liquid according to the method of Example 1, insert the suspension of the inorganic phosphorus-solving bacteria Mp1-Ha3 into the SP liquid medium containing different concentrations of soluble phosphorus according to the inoculum size of 1%, and culture it with shaking at 180 rpm and 28°C 2 days. Three replicates were set up for...

Embodiment 3

[0035] Embodiment 3: the influence of inorganic phosphorus bacterium Mp1-Ha3 on rape inoculation

[0036] The tested soil formula: 85% quartz sand, 15% montmorillonite, 2% CaCO 3 , add 500mg Ca per kg soil 3 (PO 4 ) 2 . After mixing, sterilize at 121°C for 50 min.

[0037] experimental method:

[0038] (1) Seed sterilization: 200 rapeseed seeds were soaked in sterile water for 6-7 hours, and air-dried on a clean bench. They were then soaked in 30% sodium hypochlorite solution for 90 s for surface sterilization, and then rinsed three times with sterile water.

[0039] (2) Seed germination: Thoroughly sterilized rapeseed seeds were soaked in Mp1-Ha3 bacterial suspension and normal saline respectively. After soaking for 30 min, the seeds were air-dried in an ultra-clean workbench. The air-dried rape seeds were placed on the water agar plate medium, 12 seeds were placed on each plate, and five replicates were set up for each treatment. Cultivate at 28°C in the dark, observ...

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Abstract

The invention discloses a poplar rhizosphere inorganic phosphorus-decomposing bacterium, which is Staphylococcus amberii and is classified as Staphlococcus succinus Mp1‑Ha3 has been deposited in the China Center for Type Culture Collection, accession number: CCTCC M 2019723, preservation date: September 16, 2019, preservation address: Wuhan University, Wuhan, China. The bacterium exhibits strong dissolving ability to insoluble phosphate, and is relatively stable under the induction of exogenous phosphate, and the strain has good plant growth-promoting effect.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a poplar rhizosphere inorganic phosphorus-decomposing bacterium and its application. Background technique [0002] Poplar is an important economic tree in northern Jiangsu. It has the advantages of fast growth and easy planting. However, the short-term operation mode caused a large loss of soil fertility, and the problem of soil phosphorus deficiency has gradually emerged. Phosphorus is an important nutrient required for plant growth and development, and plays an important role in the growth process of plants. However, the phosphorus source in the soil often exists in an insoluble form, which cannot be absorbed and utilized by plants. Soil phosphorus deficiency has become an important problem restricting the development of forestry. In order to meet the needs of development, a large amount of phosphorus fertilizer needs to be applied, but the appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/44
CPCA01N63/00C12N1/205C12R2001/44
Inventor 曾庆伟韩雪娇包婧婷
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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