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Animal model of breast cancer invading nerves and inducing pain and preparation method of animal model

An animal model, breast cancer technology, applied in the field of medicine, can solve the problems of slow progress and lack of research on the mechanism of nerve invasion, and achieve low cost, good stability and fast speed

Inactive Publication Date: 2021-12-17
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of a stable and effective in vivo PNI model, the research on the mechanism of nerve invasion is slow.

Method used

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  • Animal model of breast cancer invading nerves and inducing pain and preparation method of animal model
  • Animal model of breast cancer invading nerves and inducing pain and preparation method of animal model
  • Animal model of breast cancer invading nerves and inducing pain and preparation method of animal model

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Experimental program
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Effect test

Embodiment 1

[0021] 4T1 is a 6-thioguanine-resistant cell line screened from the 410.4 tumor line without mutagenesis. It can grow adherently, and its growth and metastasis characteristics in BALB / c mice are very similar to breast cancer in humans . This tumor is an animal model of human stage VI breast cancer. 4T1-induced tumors metastasized with similar kinetics after surgery and without surgery, and can be used as a postoperative and non-operative model.

[0022] In this example, the 4T1 breast cancer cell line was selected, and the tumor cells were injected subcutaneously into the sciatic nerve of mice, and the changes in the functional index and pain sensation of the sciatic nerve of the mice were observed, so as to initially establish an effective model of breast cancer invasion of nerves.

[0023] The specific process is as follows:

[0024] (1) Cell culture

[0025] After the frozen 4T1 cell line was thawed and recovered, it was placed in DMEM medium containing 10% fetal bovine ...

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Abstract

The invention discloses an animal model of breast cancer invading nerves and inducing pain and a preparation method of the animal model, and belongs to the technical field of medicines. According to the animal model of the breast cancer invading nerves and inducing pain and the preparation method of the animal model, a 4T1 breast cancer cell strain is selected, tumor cells are injected under the sciatic nerve adventitia of a mouse, and the functional index and pain change of the sciatic nerve of the mouse after injection are observed, so that an effective model of the breast cancer invading nerves is preliminarily established, and a certain experimental foundation is laid for further deeply discussing the PNI effect of the breast cancer and a pain causing mechanism; and the animal model has important significance for early diagnosis and early treatment of the breast cancer, improvement of treatment effect and alleviation of pain of clinical breast cancer patients, especially for prevention and alleviation of PMPS.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an animal model of breast cancer invading nerves and inducing pain and a preparation method thereof. Background technique [0002] In 2020, the World Health Organization's International Agency for Research on Cancer (IARC) announced that breast cancer replaced lung cancer as the most common cancer in the world. Breast cancer is a malignant tumor that seriously endangers women's health, and recurrence and metastasis are often the main reasons for its treatment failure. Neurotropic invasion (PNI) is the fifth type of metastasis after direct spread, implantation, lymphatic metastasis and hematogenous metastasis, and plays an important role in the invasion of malignant tumors. Studies have shown that PNI is closely related to the recurrence and 5-year survival rate of patients with invasive breast cancer, and is an important prognostic parameter for breast cancer. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02C12N5/071C12N5/09
CPCA01K67/02C12N5/0693C12N5/0631C12N2509/00A01K2267/03A01K2227/105
Inventor 陈霞吴坚邢佳俊张永辉魏金环陈罡
Owner NANTONG UNIVERSITY
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