Nematode extract and application thereof

An extract and nematode technology, applied in the field of pesticides, can solve the problems of high toxicity, general pine wood nematode inhibitory effect, need for further research, etc., to achieve the effect of easy acquisition and improved activity

Active Publication Date: 2021-12-17
沈阳恩柽研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active ingredients mainly include abamectin, thiazolphos, isofenphos-methyl, methamidophos, methyl bromide, aldicarb, amino-oligosaccharins, triamron, cartap, metamuc, carbofuran, and butylsulfone Carbofuran, etc. Most of the products are organophosphorus products, which are highly toxic. Most of the pesticides have a general inhibitory effect on pine xylophilus parasitic in woody plants such as Pinaceae. The fatality rate of wood nematode is less than 30%
Existing studies have shown that the ethanol extracts of Artemisia argyi, Gallopia vine, Sophora chinensis, Bovis chinensis, Herba Asarum and Adenophora aurantiae have strong poisonous activity against root-knot nematodes of bananas, and peppermint, neem, Polygonum water, pokeweed, Methanol extracts of Medicinal Plants such as Flyweed, Artemisia annua, Lianda and other medicinal plants have good poisonous activity against soybean cyst nematodes, but these extracts are only toxic and active against specific species of nematodes, and the components of active nematode active substances Its mechanism of action and practical application need to be further studied
[0010] At the same time, based on the classification, main form, reproductive mode, and most importantly Significant differences in the tolerance of pesticides or compounds can prove to a considerable extent that the same class of pesticides and / or compounds have incomparable anti-nematode effects on different nematodes
Hu Junjie (2017, East China University of Science and Technology) took the nematode-killing 5-HT3 receptor antagonist MDL-72222 as the lead, by changing the position of the aza double bridge chain, and introducing the pesticide active group thiadiazole or thiazolyl , designed and synthesized 61 unreported 3-azabicyclo[3.2.1]octane arylthiadiazole / thiazole compounds, and tested 42 of them against Meloidogyne incognita and B. xylophilus Activity experiment (nematode in vitro test), the result shows: have 6 kinds of compounds to have certain inhibitory activity to pine wood nematode in the tested compound; The compounds with inhibitory activity against pine xylophilus are different; that is, the antinematode activity of the same class or even the same compound against different nematodes is not comparable

Method used

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  • Nematode extract and application thereof
  • Nematode extract and application thereof
  • Nematode extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1, the preparation of the crude extract of pine xylophilus and its chemical composition analysis

[0117] 1 The source of the tested pine xylophilus and the expanded culture of the pine xylophilus

[0118] Weigh 300g of dry corn kernels with complete shape and soak them in water for 2 days at room temperature, then pack them into 10 Erlenmeyer flasks with a capacity of 250mL. Bacteria for 30 minutes. After the sterilization is completed, wait for it to cool naturally to room temperature, inoculate an appropriate amount of Botrytis cinerea under aseptic conditions, and culture it in an artificial climate box at 25°C and 70% RH in the dark for 5-7 days, and then pour it into each Erlenmeyer flask Inoculate 3000-5000 pine wood nematodes, and continue culturing in an artificial climate chamber at 25° C. and 70% RH in the dark for 8-15 days to harvest a large number of pine wood nematodes.

[0119] 2 Preparation of Crude Extract of Pine Xylophilus

[0120] Afte...

Embodiment 2

[0134] Embodiment 2, the activity experiment of pine wood nematode crude extract to pine wood nematode

[0135] Drug configuration: the nematode crude extract prepared in Example 1 is configured into a 10g / L aqueous solution with the DMSO test system (4%DMSO+15% Tween 80), and the DMSO test system (4%DMSO+15% Tween 80 ) was the blank control group.

[0136] Preparation of the nematode suspension: suspend the pine wood nematode on the corn grain medium with an appropriate amount of sterilized water, and then separate the living pine wood nematode suspension by the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in t...

Embodiment 3

[0141] Embodiment 3, the activity test of the active ingredient monomer contained in the pine wood nematode extract to pine wood nematode

[0142] Experiment 1. Activity and LC50 determination of azelaic acid against pine xylophilus

[0143] (1) Activity experiment of azelaic acid (DMSO / Tween water system) on pine xylophilus

[0144] Pharmacy preparation:

[0145] The preparation of the following experimental group medicaments is as follows: Azelaic acid (commercially available) is prepared through an aqueous solution containing DMSO and Tween 80 to prepare different concentrations of azelaic acid aqueous solutions; wherein, the content of DMSO in the aqueous solution is 4%, and the content of Tween 80 15%.

[0146] test group:

[0147] 1) 10 mL of azelaic acid aqueous solution with a concentration of 7 g / L;

[0148] 2) 10 mL of azelaic acid aqueous solution with a concentration of 5 g / L;

[0149] 3) 10 mL of azelaic acid aqueous solution with a concentration of 3 g / L;

...

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PUM

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Abstract

The invention belongs to the field of pesticides, and particularly relates to a nematode extract and application of an active compound of the nematode extract as a nematicide. The nematode extract is extracted by taking nematodes as raw materials, and the extract contains a series of compounds with nematicidal activity. One or more of the nematode extract, the active compound, the salt or the ester thereof can be mixed with agriculturally acceptable solvents and / or auxiliary materials to form a nematicidal preparation, and the nematicidal preparation is used as a nematicide for protecting plants against nematode infection or harm.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to a nematode extract and its application as a nematocidal preparation. Background technique [0002] Nematodes, belonging to the phylum Nematodes, can be found everywhere in fresh water, sea water, and land. It is an animal group second only to insects in diversity. Currently, there are more than 28,000 recorded species, including 256 families and 2,271 genera. There are still a large number of species that have not yet been identified. name. In 1743, after the British scientist Needham first discovered the tritici nematode in diseased wheat grains, different reports of various types of nematodes appeared successively in various regions of the world. In 1993, Yeates and other scholars divided the nutritional groups of nematodes into 8 groups according to the feeding characteristics of nematodes, the structure of the esophagus, and the method of predation, among which plant-pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/12A01N37/04A01N37/36A01N43/90A01P5/00
CPCA01N63/12A01N37/04A01N37/36A01N43/90Y02A50/30
Inventor 李世友赵福强王萍李帅强韩月泠
Owner 沈阳恩柽研究院有限公司
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