Cell high-activity staining method

Abstract

The embodiment of the invention provides a cell high-activity staining method. The method comprises the following steps: mixing a sample containing target cells with a specific direct-labeled antibody; performing electroporation marking on the target cells in the sample through an electrode, wherein in the electroporation process, the specific direct-labeled antibody enters the target cells to complete specific marking; and standing the specifically labeled target cells to restore the cell membranes of the target cells. According to the invention, in the process of introducing the specific direct-labeled antibody into the target cells by using an electroporation method, irreversible damage to the cell is not caused, and after the electroporation operation is completed, the cell membrane of the target cell can be recovered and recovered to high activity within 10 minutes, and the labeling specificity is close to 100%.

Description

technical field [0001] The invention relates to the field of biological / medical identification, in particular to a method for staining cells with high activity. Background technique [0002] Immunofluorescence staining is a technique for antigen localization by labeling antibodies with fluorescent substances, and is often used in the detection and identification of target cells. [0003] In the commonly used immunofluorescence staining technique, in order to improve the staining efficiency, a series of steps such as fixation, membrane rupture, blocking, and antibody incubation are generally required. Among them, the purpose of fixation is to terminate the activity of intracellular enzymes and other metabolic activities, and the purpose of membrane rupture is to destroy the structure of the cell membrane, expose the site of the antigen protein in the membrane, and ensure that the antigen and antibody have the opportunity to combine. The above steps are irreversible damage, s...

AI Technical Summary

Problems solved by technology

The above steps are all irreversible damage, so the cells after immunofluorescence staining are generally extremely low in activity, and downstream cell analysis cannot be realized, such as the measurement of Young's modulus of living cells, the analysis of proliferation characteristics, and the analysis of metastasis characteristics, etc.

In addition, in the traditional immunofluorescence staining technique, the whole experiment takes more than 2 hours (even overnight incubation), and the cell staining cannot be completed quickly

[0004] In summary, existing methods cannot rapidly achieve highly specific, highly active specific labeling of target cells

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