Immune tissue chemistry micro colourimeter and its making method

A technology of immunohistochemistry and colorimeters, applied in the field of colorimetric devices, can solve the problems of lack of objective reference standards for color change and limited quantitative analysis, so as to overcome subjectivity and inconsistency, express objectively and accurately, and easily The effect of making

Inactive Publication Date: 2004-02-11
申洪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no unified method and standard for computerized quantitative analysis of immunohistochemistry at present, and the quantitative indicators used often vary from person to person, and the relevant quantitative analysis is limited to research, while the analysis of a large number of immunohistochemical staining results in clinical pathology still remains to be done. Using traditional observation and judgment, there is a lack of objective reference standards for measuring color changes

Method used

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  • Immune tissue chemistry micro colourimeter and its making method
  • Immune tissue chemistry micro colourimeter and its making method
  • Immune tissue chemistry micro colourimeter and its making method

Examples

Experimental program
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Embodiment 1

[0017] Such as figure 1 As shown, the substrate of the immunohistochemical microscopic colorimeter is a circular high-transparency film with a diameter of 1.9 cm and a thickness of less than 1 mm. There is a linear transparent ribbon on the circular surface, and the ribbon consists of 10 The individual color points are sorted into ten grades from 0 to 9 according to the brownish-yellow-tan color from light to dark (0 grade is colorless). The color bands on this immunohistochemical microscopic colorimeter are made by photographing copies.

Embodiment 2

[0019] Such as figure 2 As shown, the substrate of the immunohistochemistry microscopic colorimeter is a circular highly transparent glass slide with a diameter of 2 cm and a thickness of 1 mm. There are transparent ribbons arranged in a circle by 4 color points on the circular surface. The 4 color points are sorted from light to dark according to the brownish-yellow tan color and the colorless color point in the center is divided into five grades from 0 to 4 (0 grade is colorless). The ribbons on the immunohistochemical microscopic colorimeter are directly printed on the highly transparent glass substrate by color printing technology (using transparent pigments).

Embodiment 3

[0021] Such as image 3 As shown, the substrate of the immunohistochemical microscopic colorimeter is a circular high-transparency film with a diameter of 2 cm and a thickness of less than 1 mm. There are transparent ribbons arranged in a circle by 8 color points on the circular surface, and 8 The individual color points are sorted from light to dark according to the brownish-yellow tan color, and the colorless color point in the center is divided into nine grades from 0 to 8 (grade 0 is colorless). The manufacturing method of this immunohistochemical microscopic colorimeter is to directly print the color ribbon pattern designed on the computer on the high-transparency film substrate with a color printer with transparent pigments, and then cut into immunohistochemical microscopic colorimetric colorimeters according to the required size. device.

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Abstract

An immunohistochemical microchromatometer has a transparent sheet as base body, on which there is a transparent chromatic band from light colour (tan) to dark colour (tawny), which are graduted. Said microchromatometer can be put in eye lens of microscopic to provide a colour reference to judge the immunohistochemical stain result. It can be made up by photography, copy and colour print.

Description

technical field [0001] The invention belongs to a microscope accessory, and relates to a colorimetric device for observing the color development result of a chemical reaction with a microscope. Background technique [0002] Immunohistochemical reactions were visualized in tan by DAB (3,3-dimethylaminobenzidine) chromogenic reaction. This color development can be observed through a microscope. The chromogenic results of immunohistochemical reaction can be divided into negative and positive, which are indicated by "-, +" symbols, and those between negative and positive are suspiciously positive, and are indicated by ±. Positive results can be divided into different positive grades according to the degree of positive reaction. Currently, there are grades I, II, III, and IV, which are recorded as +, ++, +++, and ++++ respectively. The division of negative and positive and the grading of positive degree are currently judged according to the color change of the color development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01J3/02G01N33/52
Inventor 申洪
Owner 申洪
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