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SNP molecular marker related to cholesterol metabolism of meat rabbits and application of SNP molecular marker

A molecular marker, cholesterol technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of small size, limited medicinal value of meat rabbits, and high cholesterol

Pending Publication Date: 2021-12-24
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, because of its strong wildness, small size, slow growth rate, and relatively high cholesterol, the medicinal value of meat rabbits is also limited.

Method used

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  • SNP molecular marker related to cholesterol metabolism of meat rabbits and application of SNP molecular marker
  • SNP molecular marker related to cholesterol metabolism of meat rabbits and application of SNP molecular marker
  • SNP molecular marker related to cholesterol metabolism of meat rabbits and application of SNP molecular marker

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Experimental program
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Embodiment 1

[0023] 1. Source of experimental animals

[0024] The experimental meat rabbit breed used in this embodiment is as follows:

[0025] Fujian Yellow Rabbit, provided by the Natural Ecological Agriculture Experimental Field in Lianjiang Yuhuashan, Fujian Province;

[0026] Southwest Fujian Black Rabbit, provided by Longyan Tongxian Rabbit Industry Development Co., Ltd.;

[0027] New Zealand white rabbits and Fujian white rabbits were provided by the Fujian White Rabbit Breeding Farm in Yuantian Village, Wudong Township, Wuping County.

[0028] 2. Determination of Cholesterol Content and Low Density Lipoprotein Content in Meat Rabbits

[0029] Collect 1-2 mL of blood from the ear margin of fasting meat rabbits and put it in a coagulation-promoting vacuum blood collection tube (the test animal was fasted for 12 hours before blood collection, and at the same time allowed it to drink water freely, and the ear vein blood collection was performed in an awake state), flick the bottom ...

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Abstract

The invention relates to an SNP molecular marker related to cholesterol metabolism of meat rabbits and application of the SNP molecular marker. The SNP molecular marker is located in a third intron region of a CAST gene of the meat rabbits, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, a G / T mutation site exists at the 190th basic group of the sequence, the mutation causes the nucleotide of the sequence to generate polymorphism, and when the genotype of the site is GG, the meat rabbits to be detected belong to individuals with low cholesterol and low-density lipoprotein content in plasma.

Description

technical field [0001] The invention belongs to the technical field of rabbit molecular marker preparation, in particular to a SNP molecular marker related to meat rabbit cholesterol metabolism and its application. Background technique [0002] Meat rabbit not only has good economic value, but also has good medicinal value. The medical treasure house "Compendium of Materia Medica" of Li Shizhen, a famous medical scientist in ancient my country, has made a comprehensive introduction to the medicinal value of rabbits. For example, rabbit meat is used as medicine, It can play a very good effect of nourishing the middle and replenishing qi, heat and dampness, quenching thirst and invigorating the spleen, and suppressing Danshi poison; rabbit blood is used as medicine, which can cool blood and activate blood, relieve fetal heat and poison, and induce labor and labor The effect of rabbit liver is used as medicine, which can play a very good effect of improving eyesight and replenish...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 桑雷孙世坤陈冬金王锦祥高晨芳谢喜平陈岩锋
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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